JMY Antibody

Datasheet

Code	CSB-PA850838LA01HU
Size	US$166
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Image	IHC image of CSB-PA850838LA01HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA850838LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) JMY Polyclonal antibody

Uniprot No.

Q8N9B5

Target Names

JMY

Alternative Names

FLJ37870 antibody; Jmy antibody; JMY protein antibody; JMY_HUMAN antibody; junction mediating and regulatory protein antibody; Junction mediating and regulatory protein p53 cofactor antibody; Junction-mediating and -regulatory protein antibody; MGC163496 antibody; OTTHUMP00000161976 antibody; WAS protein homology region 2 domain containing 1-like 3 antibody; WHDC1L3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Junction-mediating and -regulatory protein (708-848AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The JMY Antibody (Product code: CSB-PA850838LA01HU) is Non-conjugated. For JMY Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA850838LB01HU	JMY Antibody, HRP conjugated	ELISA
FITC	CSB-PA850838LC01HU	JMY Antibody, FITC conjugated
Biotin	CSB-PA850838LD01HU	JMY Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.

Gene References into Functions

In autophagosomes, the integrity of the WH2 domains allows JMY to promote actin nucleation, which is required for efficient autophagosome formation. PMID: 26223951
JMY was expressed in normal tissues and heterogeneously in different tumor types, with close correlation between cytoplasmic and nuclear expression. PMID: 25280461
JARID1A, JMY, and PTGER4 polymorphisms are related to ankylosing spondylitis in Chinese Han patients. PMID: 24069348
JMY is related to the severity of AS in Chinese Han patients. PMID: 23758122
actin assembly regulates nuclear import of JMY in response to DNA damage. PMID: 22262458
JMY is expressed at high levels in brain tissue, and in various cell lines JMY is predominantly cytoplasmic, with a minor fraction in the nucleus. PMID: 21965285
results establish the interplay between JMY and HIF-1alpha as a new mechanism that controls cell motility under hypoxic stress PMID: 21625218
Data demonstrate a pathway that links the cytoskeleton with the p53 response, and further suggest that the ability of JMY to regulate actin and cadherin is instrumental in coordinating cell motility with the p53 response. PMID: 19897726
JMY represents a new class of multifunctional actin assembly factor whose activity is regulated, at least in part, by sequestration in the nucleus. PMID: 19287377

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton.

Protein Families

JMY family

Database Links

HGNC: 28916

OMIM: 604279

KEGG: hsa:133746

STRING: 9606.ENSP00000379441

UniGene: Hs.482605

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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