KIF22 Antibody

Datasheet

Code	CSB-PA619894LA01HU
Size	US$166
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Image	IHC image of CSB-PA619894LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of NIH/3T3 cells with CSB-PA619894LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) KIF22 Polyclonal antibody

Uniprot No.

Q14807

Target Names

KIF22

Alternative Names

A 328A3.2 antibody; KID antibody; KIF 22 antibody; kif22 antibody; KIF22_HUMAN antibody; Kinesin family member 22 antibody; Kinesin like 4 antibody; Kinesin like DNA binding protein antibody; Kinesin like DNA binding protein pseudogene antibody; Kinesin like protein 4 antibody; Kinesin like protein KIF22 antibody; Kinesin-like DNA-binding protein antibody; Kinesin-like protein 4 antibody; Kinesin-like protein KIF22 antibody; KNSL 4 antibody; KNSL4 antibody; OBP 1 antibody; OBP 2 antibody; OBP antibody; OBP1 antibody; OBP2 antibody; Origin of plasmid DNA replication binding protein antibody; OriP binding protein antibody; OTTHUMP00000123406 antibody; SEMDJL2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Kinesin-like protein KIF22 protein (34-169AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The KIF22 Antibody (Product code: CSB-PA619894LA01HU) is Non-conjugated. For KIF22 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA619894LB01HU	KIF22 Antibody, HRP conjugated	ELISA
FITC	CSB-PA619894LC01HU	KIF22 Antibody, FITC conjugated
Biotin	CSB-PA619894LD01HU	KIF22 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA. Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells.

Gene References into Functions

Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules. PMID: 25743205
we conclude that inhibition of KIF22 suppresses cancer cell proliferation by delaying mitotic exit through the transcriptional upregulation of CDC25C. PMID: 24626146
Whole-exome sequencing identifies mutations of KIF22 in spondyloepimetaphyseal dysplasia with joint laxity, leptodactylic type PMID: 22152677
Recurrent dominant mutations affecting two adjacent residues in the motor domain of the monomeric kinesin KIF22 result in skeletal dysplasia and joint laxity PMID: 22152678
in all breast tumor tissues analyzed, variations in the Kid/KIF22 mRNA levels mirrored those seen with SIAH-1 mRNAs. PMID: 20144232
These results are consistent with Kid having a role in chromosome congression in vivo, where it would be responsible for the polar ejection forces acting on the chromosome arms. PMID: 12606572
its molecular structure and interaction with microtubules PMID: 12692123
These results suggest that distinct from its role in chromosome movement, Kid contributes to spindle morphogenesis by mediating spindle microtubules stabilization. PMID: 16176979
human Aurora B and Kid are identified as APC/C(Cdh1) substrates PMID: 17726374
Association of importin-beta and -alpha with hKid triggers the initial targeting of hKid to mitotic chromosomes; local Ran-GTP-mediated cargo release promotes the accumulation of hKid on chromosomes. PMID: 18268099
These data suggest that Kid-mediated anaphase/telophase chromosome compaction prevents formation of multinucleated cells. PMID: 18329364

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Involvement in disease

Spondyloepimetaphyseal dysplasia with joint laxity, 2 (SEMDJL2)

Subcellular Location

Nucleus. Cytoplasm, cytoskeleton.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family

Tissue Specificity

Expressed in bone, cartilage, joint capsule, ligament, skin, and primary cultured chondrocytes.

Database Links

HGNC: 6391

OMIM: 603213

KEGG: hsa:3835

STRING: 9606.ENSP00000160827

UniGene: Hs.612151

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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