LAPTM4A Antibody

Datasheet

Code	CSB-PA621858LA01HU
Size	US$166
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Image	IHC image of CSB-PA621858LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA621858LA01HU diluted at 1:300 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA621858LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LAPTM4A Polyclonal antibody

Uniprot No.

Q15012

Target Names

LAPTM4A

Alternative Names

LAPTM4A; KIAA0108; LAPTM4; MBNT; MTRP; UNQ1846/PRO3574; Lysosomal-associated transmembrane protein 4A; Golgi 4-transmembrane-spanning transporter MTP

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Lysosomal-associated transmembrane protein 4A protein (181-233AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LAPTM4A Antibody (Product code: CSB-PA621858LA01HU) is Non-conjugated. For LAPTM4A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA621858LB01HU	LAPTM4A Antibody, HRP conjugated	ELISA
FITC	CSB-PA621858LC01HU	LAPTM4A Antibody, FITC conjugated
Biotin	CSB-PA621858LD01HU	LAPTM4A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

May function in the transport of nucleosides and/or nucleoside derivatives between the cytosol and the lumen of an intracellular membrane-bound compartment.

Gene References into Functions

hOCT2 interacts with LAPTM4A in lysosomes and late endosomes and regulates endocytotic recruitment. PMID: 21553234

Subcellular Location

Endomembrane system; Multi-pass membrane protein.

Protein Families

LAPTM4/LAPTM5 transporter family

Database Links

HGNC: 6924

KEGG: hsa:9741

STRING: 9606.ENSP00000175091

UniGene: Hs.467807

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Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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