LEMD1 Antibody

Datasheet

Code	CSB-PA715035LA01HU
Size	US$166
	Order now
Image	IHC image of CSB-PA715035LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA715035LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Latest Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LEMD1 Polyclonal antibody

Uniprot No.

Q68G75

Target Names

LEMD1

Alternative Names

LEMD1; LEM domain-containing protein 1; Cancer/testis antigen 50; CT50; LEM domain protein 1; LEMP-1

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human LEM domain-containing protein 1 protein (44-151AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LEMD1 Antibody (Product code: CSB-PA715035LA01HU) is Non-conjugated. For LEMD1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA715035LB01HU	LEMD1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA715035LC01HU	LEMD1 Antibody, FITC conjugated
Biotin	CSB-PA715035LD01HU	LEMD1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Gene References into Functions

Data show that LEM domain containing 1 (LEMD1)has five transcript splicing variants (variant 1 [V1] - variant 5 [V5]), and that LEMD1 V1, V2 and V3 is expressed in testis and CR-CSCs/CICs, whereas LEMD1 V4 and V5 is ubiquitously expressed. PMID: 28219643
Downregulation of LEMD1 expression inhibited adhesion and transmigration of OSCCs. PMID: 27280633
Of the prostate cancer samples, 10, 10, 23 and 40% showed ODF1, ODF2, LEMD1 and SPATA19 specific bands, respectively, but none of the BPH samples expressed any of these genes. PMID: 20682177
LEM domain-containing 1 is a novel testis-specific gene expressed in colorectal cancers PMID: 15254688

Subcellular Location

Membrane; Single-pass membrane protein.

Tissue Specificity

Testis-specific. Isoform 6 is detected in 17 of 18 colon cancer tissues examined.

Database Links

HGNC: 18725

OMIM: 610480

KEGG: hsa:93273

STRING: 9606.ENSP00000356121

UniGene: Hs.655520

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address