MAF1 Antibody

Datasheet

Code	CSB-PA872421HA01HU
Size	US$166
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Image	Immunofluorescence staining of Hela cell with CSB-PA872421HA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of MCF-7 cell with CSB-PA872421HA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MAF1 Polyclonal antibody

Uniprot No.

Q9H063

Target Names

MAF1

Alternative Names

Homolog of yeast MAF1 antibody; maf1 antibody; MAF1 homolog (S. cerevisiae) antibody; MAF1 homolog antibody; MAF1_HUMAN antibody; MGC20332 antibody; MGC31779 antibody; MGC39758 antibody; Repressor of RNA polymerase III transcription MAF1 homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Repressor of RNA polymerase III transcription MAF1 homolog protein (1-256AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MAF1 Antibody (Product code: CSB-PA872421HA01HU) is Non-conjugated. For MAF1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA872421HB01HU	MAF1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA872421HC01HU	MAF1 Antibody, FITC conjugated
Biotin	CSB-PA872421HD01HU	MAF1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:20-1:100

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance. Plays also a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation. Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB. When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout. Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP.

Gene References into Functions

can act as a transcriptional activator for PTEN, which is important for MAF1's tumor-suppressor function PMID: 26910647
Repression of Pol III recruitment and transcription are tightly linked to MAF1, which selectively localizes at Pol III loci, even under serum-replete conditions, and increasingly targets transcribing Pol III in response to serum starvation PMID: 26941251
These results reveal a novel mechanism by which MAF1 and Pol III regulate the activity of the CDKN1A-encoding gene transcribed by Pol II. PMID: 26067234
novel role for Maf1 in suppressing both lipid biogenesis and tumor formation; Maf1 elicits these biological responses through its ability to repress genes that that synthesize lipids and regulate biosynthetic capacity. PMID: 25502566
Studies on Maf1 regulation up until now have focused on posttranslational mechanisms, notably phosphorylation, which controls Maf1 localization (in yeast) and its interaction with the polymerase (in yeast and humans) PMID: 25568945
SUMOylation has a role in controlling Maf1 and RNA pol III-mediated transcription PMID: 23673667
Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation. PMID: 22810236
The TOR pathway controls another aspect of Pol III transcription that is closely linked to MAF1, as it depends on the presence of MAF1. PMID: 21428925
an important role of CK2-mediated Maf1 phosphorylation in triggering Pol III activation. PMID: 21383183
Data relate Pol III transcription inhibition to Maf1 structural changes. PMID: 20817737
Maf1, a repressor that binds and inhibits pol III, is phosphorylated in a mTOR-dependent manner both in vitro and in vivo at serine 75, a site that contributes to its function as a transcriptional inhibitor. PMID: 20543138
mTOR inhibition led to an increase in the occupancy of Maf1 on a set of Pol III-dependent genes, with concomitant reduction in the binding of Pol III and Brf1 PMID: 20233713
Maf1 can be co-immunoprecipitated with pol III and associates in vitro with two pol III subunits, the largest subunit RPC1 and the alpha-like subunit RPAC2. Maf1 represses pol III transcription in vitro and in vivo. PMID: 17205138
The human Maf1 protein negatively regulates transcription by all three nuclear Pols. Changes in Maf1 expression affect Pol I- and Pol III-dependent transcription in human glioblastoma lines. PMID: 17499043
represses RNA polymerase III transcription via TFIIIB, specifically through the TFIIB family members Brf1 and Brf2 PMID: 17505538
Functional studies of the yeast counterpart. PMID: 11438659

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

MAF1 family

Database Links

HGNC: 24966

OMIM: 610210

KEGG: hsa:84232

STRING: 9606.ENSP00000318604

UniGene: Hs.19673

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