MBNL3 Antibody

Datasheet

Code	CSB-PA868322LA01HU
Size	US$166
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Image	IHC image of CSB-PA868322LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA868322LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MBNL3 Polyclonal antibody

Uniprot No.

Q9NUK0

Target Names

MBNL3

Alternative Names

A530038J18Rik antibody; AI661274 antibody; CHCR antibody; Cys3His CCG1 required protein antibody; Cys3His CCG1-required protein antibody; E430034C16Rik antibody; MBLX antibody; MBLX39 antibody; MBNL 3 antibody; Mbnl3 antibody; MBNL3_HUMAN antibody; MBXL antibody; Muscleblind like 3 antibody; Muscleblind like protein 3 antibody; Muscleblind like splicing regulator 3 antibody; Muscleblind like X linked protein antibody; Muscleblind-like protein 3 antibody; Muscleblind-like X-linked protein antibody; OTTMUSP00000019072 antibody; OTTMUSP00000019073 antibody; OTTMUSP00000019074 antibody; Protein HCHCR antibody; RP23-98P13.1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Muscleblind-like protein 3 protein (116-211AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MBNL3 Antibody (Product code: CSB-PA868322LA01HU) is Non-conjugated. For MBNL3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA868322LB01HU	MBNL3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA868322LC01HU	MBNL3 Antibody, FITC conjugated
Biotin	CSB-PA868322LD01HU	MBNL3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.

Gene References into Functions

The MBNL3 splicing factor promotes hepatocellular carcinoma by increasing pacillin expression through the alternative splicing of lncRNA-PXN-AS1. PMID: 28553938
results suggest that CHCR is an inhibitor of myogenesis PMID: 12297108
MBNL proteins promote opposite splicing patterns for cardiac troponin T and insulin receptor alternative exons PMID: 15257297
We exclude MBNL3 as the causative gene in this family. PMID: 17102799
MBNL3 was not found in human samples. PMID: 19095965

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Subcellular Location

Nucleus. Cytoplasm. Note=Greater concentration in the nucleus. In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts.

Protein Families

Muscleblind family

Tissue Specificity

Highly expressed in the placenta.

Database Links

HGNC: 20564

OMIM: 300413

KEGG: hsa:55796

STRING: 9606.ENSP00000359890

UniGene: Hs.105134

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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