MED20 Antibody

Datasheet

Code	CSB-PA862046LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA862046LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA862046LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA862046LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MED20 Polyclonal antibody

Uniprot No.

Q9H944

Target Names

MED20

Alternative Names

MED20 antibody; TRFP antibody; Mediator of RNA polymerase II transcription subunit 20 antibody; Mediator complex subunit 20 antibody; TRF-proximal protein homolog antibody; hTRFP antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Mediator of RNA polymerase II transcription subunit 20 protein (1-212AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MED20 Antibody (Product code: CSB-PA862046LA01HU) is Non-conjugated. For MED20 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA862046LB01HU	MED20 Antibody, HRP conjugated	ELISA
FITC	CSB-PA862046LC01HU	MED20 Antibody, FITC conjugated
Biotin	CSB-PA862046LD01HU	MED20 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene References into Functions

Our analyses revealed no apparent differences in protein expression profiles of SRBs in central and peripheral regions of human donor tissues, indicating that carotenoid-binding proteins rather than transporters are likely to mediate selective accumulation of carotenoids into the macula. PMID: 28947101
We identified the first and homozygous mutation (p.Gly114Ala) in the Mediator subunit 20 gene (MED20) in siblings presenting with infantile-onset spasticity and childhood-onset dystonia, progressive basal ganglia degeneration, and brain atrophy PMID: 25446406

Subcellular Location

Nucleus.

Protein Families

Mediator complex subunit 20 family

Database Links

HGNC: 16840

OMIM: 612915

KEGG: hsa:9477

STRING: 9606.ENSP00000265350

UniGene: Hs.278434

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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