MID1IP1 Antibody

Datasheet

Code	CSB-PA873607LA01HU
Size	US$166
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Image	IHC image of CSB-PA873607LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA873607LA01HU diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA873607LA01HU at 1:125, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MID1IP1 Polyclonal antibody

Uniprot No.

Q9NPA3

Target Names

MID1IP1

Alternative Names

3110038L01Rik antibody; FLJ10386 antibody; G12 like antibody; Gastrulation specific G12 like protein antibody; Gastrulation-specific G12-like protein antibody; M1IP1_HUMAN antibody; MGC72582 antibody; MID1 interacting G12 like protein antibody; MID1 interacting protein 1 (gastrulation specific G12 like) antibody; Mid1-interacting G12-like protein antibody; Mid1-interacting protein 1 antibody; Mid1ip1 antibody; MIG12 antibody; OTTMUSP00000018143 antibody; OTTMUSP00000018222 antibody; OTTMUSP00000018284 antibody; OTTMUSP00000018285 antibody; Protein STRAIT11499 antibody; Protein STRAIT11499 homolog antibody; RP23-130J1.1 antibody; S14R antibody; Slap antibody; Spot 14 like androgen inducible protein antibody; Spot 14 related protein antibody; Spot 14-R antibody; Spot 14-related protein antibody; STRAIT11499 antibody; THRSPL antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Mid1-interacting protein 1 protein (1-183AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MID1IP1 Antibody (Product code: CSB-PA873607LA01HU) is Non-conjugated. For MID1IP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA873607LB01HU	MID1IP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA873607LC01HU	MID1IP1 Antibody, FITC conjugated
Biotin	CSB-PA873607LD01HU	MID1IP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules.

Gene References into Functions

The results of the present study indicated that Spot14/S14R were differently expressed in MSC adipogenesis in AIS patients, and they may be important in the abnormal adipogenic differentiation in AIS. PMID: 27082501
show that MIG12, a 22 kDa cytosolic protein of previously unknown function, binds to acetyl-CoA carboxylase and lowers the threshold for citrate activation into the physiological range. PMID: 20457939

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton.

Protein Families

SPOT14 family

Database Links

HGNC: 20715

OMIM: 300961

KEGG: hsa:58526

STRING: 9606.ENSP00000338706

UniGene: Hs.522605

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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