Name: NEGR1 Antibody
Brand: CUSABIO
SKU: CSB-PA015692LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NEGR1 Polyclonal antibody

Uniprot No.

Q7Z3B1

Target Names

NEGR1

Alternative Names

NEGR1 antibody; IGLON4 antibody; UNQ2433/PRO4993 antibody; Neuronal growth regulator 1 antibody; IgLON family member 4 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Neuronal growth regulator 1 protein (1-196AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NEGR1 Antibody (Product code: CSB-PA015692LA01HU) is Non-conjugated. For NEGR1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015692LB01HU	NEGR1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA015692LC01HU	NEGR1 Antibody, FITC conjugated
Biotin	CSB-PA015692LD01HU	NEGR1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The NEGR1 polyclonal antibody is raised in rabbits against the synthetic peptide responding to the amino acids 1-196 of the recombinant human NEGR1. It underwent protein G affinity chromatography purification and got a purity of 95%⁺. This NEGR1 antibody only reacts with human NEGR1 protein and has got quality verification in ELISA, WB, IHC, and IF applications. The recognition of the NEGR1 protein by this NEGR1 polyclonal antibody in multiple assays makes it valuable in investigating the function of the NEGR1 protein in experimental contexts.

Target Background

Function

May be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain.

Gene References into Functions

Our results indicate a possible contribution of CNVs in LEPR, NEGR1, ARHGEF4, and CPXCR1 and the intergenic regions 12q15c, 15q21.1a, and 22q11.21d to the development of obesity, particularly abdominal obesity in Mexican children. PMID: 28428959
NEGR1, strong candidate gene, associated with clinical features of Depression (e.g., earlier age at onset and recurrent and more severe forms of Depression). Gene expression patterns in the prefrontal and anterior cingulate cortex most closely matched the genetic findings. PMID: 28969442
Single nucleotide polymorphism in NEGR1 gene is associated with major depressive disorder. PMID: 27479909
Significant associations were identified at 3.5 years old for TMEM18 rs6548238, NEGR1 rs2815752, BDNF rs10767664 and rs6265 (1 year old and 3.5 years old) with anthropometric phenotypes PMID: 27005443
NEGR1 interacts with NPC2 and increases its protein stability; it has a role in obesity PMID: 27940359
Genetic variants associated with BMI and WHR in adults influence growth patterns and general and abdominal fat development from early childhood onwards. PMID: 25640768
We observed novel selection signals in CDKAL1 and NEGR1, well-known diabetes and obesity susceptibility genes PMID: 25370040
NEGR1 could be an important locus influencing certain personality dimensions in BN patients. PMID: 25245582
First study to find an association between an obesity risk gene and differences in white matter integrity. As our subjects were young and healthy, our results suggest that NEGR1 has effects on brain structure independent of its effect on obesity. PMID: 25072390
Data indicate strong associations with severe obesity for single nucleotide polymorphism (SNP) rs9939609 within the FTO gene and SNP rs2815752 near the NEGR1 gene. PMID: 23950990
Results imply a regulatory role for TMEM18, BDNF, MTCH2 and NEGR1 in adipocyte differentiation and biology. In addition, we show a variation of MAF expression during adipogenesis, while NPC1, PTER and SH2B1 were not regulated. PMID: 23229156
Our study supports earlier reports of SH2B1 to be of importance in insulin sensitivity and, in addition, suggests potential roles of NEGR1 and MTCH2. PMID: 22443470
Meta-analysis of 4992 subjects revealed seven SNPs near four loci, including NEGR1, TMEM18, SH2B1 /ATP2A1 and MC4R, showing significant association at 0.005 PMID: 21750520
Gene-treatment interactions were observed for long-term (NEGR1 rs2815752, Pmetformin*SNP = 0.028; FTO rs9939609, Plifestyle*SNP = 0.044) weight loss. PMID: 22179955
A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration. PMID: 22360420
Data suggest that in addition to its reported role in the brain, NEGR1 is also expressed in subcutaneous adipose tissue and acts as a central 'hub' in an obesity-related transcript network. PMID: 21427694
The results of these studies provide supporting evidence for MYEOV and NEGR1 as gene targets of 11q13 gains and 1p31 deletions in a neuroblastoma subset. PMID: 21624008
Observational study of gene-disease association. (HuGE Navigator) PMID: 20712903
Observational study of gene-disease association. (HuGE Navigator) PMID: 20724581
Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20725061
Observational study of gene-disease association. (HuGE Navigator) PMID: 20816152
Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20571754
Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
Observational study of gene-disease association. (HuGE Navigator) PMID: 20816195
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20386550
Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20520848
Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20215397
Observational study of gene-disease association. (HuGE Navigator) PMID: 19746409
Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 19812171
Observational study of gene-disease association. (HuGE Navigator) PMID: 19851340
Observational study of gene-disease association. (HuGE Navigator) PMID: 19910938
Observational study of gene-disease association. (HuGE Navigator) PMID: 19692490
Observational study of gene-disease association. (HuGE Navigator) PMID: 19164386
Observational study, meta-analysis, and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19079261

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Families

Immunoglobulin superfamily, IgLON family

Database Links

HGNC: 17302

OMIM: 613173

KEGG: hsa:257194

STRING: 9606.ENSP00000350364

UniGene: Hs.146542

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Code	CSB-PA015692LA01HU
Size	US$166
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Image	Western blot All lanes: NEGR1 antibody at 4µg/ml + U87 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 25 kDa Observed band size: 25 kDa Immunohistochemistry analysis of human skeletal muscle tissue using CSB-PA015692LA01HU at dilution of 1:100 Immunohistochemistry analysis of human testis tissue using CSB-PA015692LA01HU at dilution of 1:100 Immunofluorescent analysis of PC-3 cells using CSB-PA015692LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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NEGR1 Antibody

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