NEK10 Antibody

Datasheet

Code	CSB-PA747816LA01HU
Size	US$166
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Image	IHC image of CSB-PA747816LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA747816LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NEK10 Polyclonal antibody

Uniprot No.

Q6ZWH5

Target Names

NEK10

Alternative Names

NEK10 antibody; Serine/threonine-protein kinase Nek10 antibody; EC 2.7.11.1 antibody; Never in mitosis A-related kinase 10 antibody; NimA-related protein kinase 10 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Serine/threonine-protein kinase Nek10 protein (1-104AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NEK10 Antibody (Product code: CSB-PA747816LA01HU) is Non-conjugated. For NEK10 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA747816LB01HU	NEK10 Antibody, HRP conjugated	ELISA
FITC	CSB-PA747816LC01HU	NEK10 Antibody, FITC conjugated
Biotin	CSB-PA747816LD01HU	NEK10 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Plays a role in the cellular response to UV irradiation. Mediates G2/M cell cycle arrest, MEK autoactivation and ERK1/2-signaling pathway activation in response to UV irradiation. In ciliated cells of airways, it is involved in the regulation of mucociliary transport.

Gene References into Functions

Nek10 physically associated with Raf-1 and MEK1 in a Raf-1-dependent manner, and the formation of this complex was necessary for Nek10-mediated MEK1 activation. PMID: 20956560

Protein Families

Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily

Tissue Specificity

Expressed in the lung.

Database Links

HGNC: 18592

KEGG: hsa:152110

STRING: 9606.ENSP00000343847

UniGene: Hs.506115

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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