NEURL1B Antibody

Datasheet

Code	CSB-PA015722LA01HU
Size	US$166
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Image	IHC image of CSB-PA015722LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA015722LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA015722LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NEURL1B Polyclonal antibody

Uniprot No.

A8MQ27

Target Names

NEURL1B

Alternative Names

E3 ubiquitin-protein ligase NEURL1B antibody; E3 ubiquitin-protein ligase NEURL3 antibody; hNeur2 antibody; NEU1B_HUMAN antibody; Neur2 antibody; neuralized-2 alternative protein antibody; neuralized-2 antibody; Neuralized-like protein 1B antibody; Neuralized-like protein 3 antibody; NEURL1B antibody; NEURL3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human E3 ubiquitin-protein ligase NEURL1B protein (1-275AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NEURL1B Antibody (Product code: CSB-PA015722LA01HU) is Non-conjugated. For NEURL1B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015722LB01HU	NEURL1B Antibody, HRP conjugated	ELISA
FITC	CSB-PA015722LC01HU	NEURL1B Antibody, FITC conjugated
Biotin	CSB-PA015722LD01HU	NEURL1B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

E3 ubiquitin-protein ligase involved in regulation of the Notch pathway through influencing the stability and activity of several Notch ligands.

Gene References into Functions

Neur2, in contrast to Neur1, is highly expressed during embryonic development of the brain and several non-neural tissues and its mRNA levels subside postnatally. PMID: 19723503

Subcellular Location

Cytoplasm.

Tissue Specificity

Highest expression in brain, prostate and small intestine. In the brain the levels are higher in fetal than in adult stage. In the adult brain the highest levels are detected in the olfactory system, cerebellar cortex, optic nerve and the frontal lobe.

Database Links

HGNC: 35422

OMIM: 615893

KEGG: hsa:54492

STRING: 9606.ENSP00000358815

UniGene: Hs.91521

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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