OPN1SW Antibody

Datasheet

Citations (1)

Code	CSB-PA016354LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: U87 whole cell lysate All lanes: OPN1SW antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa Immunofluorescence staining of U251 cells with CSB-PA016354LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) OPN1SW Polyclonal antibody

Uniprot No.

P03999

Target Names

OPN1SW

Alternative Names

OPN1SW; BCP; Short-wave-sensitive opsin 1; Blue cone photoreceptor pigment; Blue-sensitive opsin; BOP

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Short-wave-sensitive opsin 1 protein (307-348AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The OPN1SW Antibody (Product code: CSB-PA016354LA01HU) is Non-conjugated. For OPN1SW Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA016354LB01HU	OPN1SW Antibody, HRP conjugated	ELISA
FITC	CSB-PA016354LC01HU	OPN1SW Antibody, FITC conjugated
Biotin	CSB-PA016354LD01HU	OPN1SW Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Deciphering the pathogenesis of retinopathy associated with carnitine palmitoyltransferase I deficiency in zebrafish model ZS Ulhaq,Biochemical and biophysical research communications,2023

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : immunohistochemistry

Sample type: cells

Review: OPN1SW antibodies, which are the rod OS, green/red cones OS, and blue cone markers, respectively. In the MO-injected larvae, the expressions of Rho, ZPR1, and OPN1SW were dramatically suppressed.

By Anonymous

Target Background

Function

Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. Required for the maintenance of cone outer segment organization in the ventral retina, but not essential for the maintenance of functioning cone photoreceptors. Involved in ensuring correct abundance and localization of retinal membrane proteins. May increase spectral sensitivity in dim light.

Gene References into Functions

LVAVA haplotype of the OPN1LW gene and MVAVA haplotype of the OPN1MW gene cause apparently nonsyndromic high myopia in young patients but lead to progressive cone-rod dystrophy with deuteranopia and protanopia in middle-aged patients corresponding to a previously unknown disease course. PMID: 28358949
Data suggest that insights into dimerization interface of red cone opsin should aid investigations of the structure and function of GPCR cell signaling. PMID: 28045251
A novel homozygous PDE6C mutation was identified as the cause of ACHM. In addition, we identified an OPN1SW mutation in the sibling with complete achromatopsia. PMID: 25605338
Individuals with the T190I S-opsin mutation behaved as mild tritans at 12.3-92.3Td, but as tritanopes at 1.2-9.2Td, for both light-adapted and dark-adapted conditions. The results are consistent with the mutant opsin causing abnormal S-cone function. PMID: 23022137
A novel mutation(prolin/leucine) in the short-wavelength-sensitive cone pigment gene associated with a tritan color vision defect. PMID: 16961973
Results show that, although light absorption behaves differently in blue, green and red opsins, their low-frequency vibrational motions are similar. PMID: 19189139
11-cis-retinol had no significant effect on the activity of human blue cone opsin PMID: 19386593
Immunoreactivity to anti-OPN1SW antibodies was seen in the upper layer of human epidermis & reconstructed skin. The opsin mRNA was seen in total RNA from human skin. Neither immunoreactivity nor mRNA expression was seen in cultured human keratinocytes. PMID: 19493002

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Involvement in disease

Tritan color blindness (CBT)

Subcellular Location

Cell membrane; Multi-pass membrane protein. Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment. Cytoplasm, perinuclear region.

Protein Families

G-protein coupled receptor 1 family, Opsin subfamily

Tissue Specificity

The three color pigments are found in the cone photoreceptor cells. Expressed throughout the epidermis and dermis, primarily in the stratum granulosum in the facial and abdominal skin (at protein level). Expressed in dermal fibroblasts (at protein level).

Database Links

HGNC: 1012

OMIM: 190900

KEGG: hsa:611

STRING: 9606.ENSP00000249389

UniGene: Hs.656404

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

G protein coupled receptor signaling pathway Target Therapy

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