ORF1 Antibody

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Datasheet

Code	CSB-PA327230LA01HGE
Size	US$166
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Target Background

Product Details

Full Product Name

Rabbit anti-Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1) ORF1 Polyclonal antibody

Uniprot No.

P33424

Target Names

ORF1

Alternative Names

ORF1 antibody; Non-structural polyprotein pORF1 [Includes: Methyltransferase antibody; EC 2.1.1.- antibody; EC 2.7.7.-); Putative papain-like cysteine protease antibody; PLP antibody; EC 3.4.22.-); NTPase/helicase antibody; EC 3.6.4.-); RNA-directed RNA polymerase antibody; RdRp antibody; EC 2.7.7.48)] antibody

Raised in

Rabbit

Species Reactivity

Hepatitis E virus genotype 1

Immunogen

Recombinant Hepatitis E virus genotype 1 Non-structural polyprotein pORF1 protein (60-240AA)

Immunogen Species

Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1)

Conjugate

Non-conjugated

The ORF1 Antibody (Product code: CSB-PA327230LA01HGE) is Non-conjugated. For ORF1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA327230LB01HGE	ORF1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA327230LC01HGE	ORF1 Antibody, FITC conjugated
Biotin	CSB-PA327230LD01HGE	ORF1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Methyltransferase: Displays a capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m(7)GTP or m(7)GDP. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m(7)GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure.; Papain-like cysteine protease: May participate in the processing of polyprotein pORF1 together with cellular proteases and the cleavage of capsid protein ORF2.; NTPase/helicase: Multi-functional protein that exhibits NTPase and RNA unwinding activities. Hydrolyzes all NTPs efficiently and unwinds RNA duplexes containing 5' overhangs. Possesses a sequence independent RNA-5'-triphosphatase (RTPase) activity suggestive of its role in forming viral cap structure. Participates also in viral genome replication, RNA translocation and genome packaging/unpackaging.; RNA-directed RNA polymerase: Plays an essential role in the virus replication. Binds to the 3'-end of the genomic RNA to initiate viral replication.

Subcellular Location

Host cytoplasm.

Protein Families

Hepevirus non-structural polyprotein family

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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