PAFAH2 Antibody

Datasheet

Code	CSB-PA857448LA01HU
Size	US$166
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Image	IHC image of CSB-PA857448LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA857448LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PAFAH2 Polyclonal antibody

Uniprot No.

Q99487

Target Names

PAFAH2

Alternative Names

PAFAH2Platelet-activating factor acetylhydrolase 2 antibody; cytoplasmic antibody; EC 3.1.1.47 antibody; Serine-dependent phospholipase A2 antibody; SD-PLA2 antibody; hSD-PLA2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Platelet-activating factor acetylhydrolase 2, cytoplasmic protein (1-206AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PAFAH2 Antibody (Product code: CSB-PA857448LA01HU) is Non-conjugated. For PAFAH2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA857448LB01HU	PAFAH2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA857448LC01HU	PAFAH2 Antibody, FITC conjugated
Biotin	CSB-PA857448LD01HU	PAFAH2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the hydrolyze of the acetyl group at the sn-2 position of platelet-activating factor (PAF) and its analogs, leading to their inactivation. Hydrolyzes propionyl and butyroyl moieties approximately half as effectively as PAF. Also catalyzes transacetylation of the acetyl group from platelet-activating factor (PAF) to lysoplasmalogen and to sphingosine, producing plasmalogen analogs of PAF and N-acetylsphingosine (C2-ceramide) respectively. Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position.

Gene References into Functions

Data show that the activity of plasma platelet activating factor acetylhydrolase (PAF-AH) increases in parallel with diabetic retinopathy (DR) severity. PMID: 26791393
The oligomeric state of PAFAH-II drives functional protein trafficking. PAFAH-II localization to the membrane is critical for substrate acquisition and effective oxidative stress protection. PMID: 25707358
presence of platelet-activating factor acetylhydrolase II in a restricted pattern in human skin; it is involved in protecting this organ against oxidative stress through the degradation of oxidatively modified bioactive phospholipids. PMID: 12406338
Lp-PLA2 was higher in those with more extensive Coronary artery disease PMID: 17157859
PAF-AH II exerts strong neuroprotective effects against ischemic injury: the neurological deficit scores, cerebral edema index, and relative infarction volume are all significantly lower in PAFAH2 transgenic mice than in wild-type mice. PMID: 17272759
examines substrate specificity for intracellular and plasma PAF-AHs PMID: 11294621

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Subcellular Location

Cytoplasm. Membrane; Lipid-anchor. Endoplasmic reticulum membrane; Lipid-anchor.

Protein Families

Serine esterase family

Tissue Specificity

Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.

Database Links

HGNC: 8579

OMIM: 602344

KEGG: hsa:5051

STRING: 9606.ENSP00000363400

UniGene: Hs.477083

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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