POU4F1 Antibody

Datasheet

Code	CSB-PA22749A0Rb
Size	US$166
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Image	Immunofluorescence staining of Hela cells with CSB-PA22749A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate All lanes: POU4F1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 34 kDa Observed band size: 43 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) POU4F1 Polyclonal antibody

Uniprot No.

Q01851

Target Names

POU4F1

Alternative Names

Brain specific homeobox/POU domain protein 3A antibody; Brain-3A antibody; Brain-specific homeobox/POU domain protein 3A antibody; Brn 3.0 antibody; BRN 3A antibody; Brn-3A antibody; Brn3 antibody; BRN3A antibody; class 4 antibody; FLJ13449 antibody; Homeobox/POU domain protein RDC 1 antibody; Homeobox/POU domain protein RDC-1 antibody; Homeobox/POU domain protein RDC1 antibody; Oct T1 antibody; Oct-T1 antibody; OctT1 antibody; PO4F1_HUMAN antibody; POU class 4 homeobox 1 antibody; POU domain antibody; POU Domain Class 4 Transcription Factor 1 antibody; Pou4f1 antibody; RDC 1 antibody; RDC1 antibody; transcription factor 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human POU domain, class 4, transcription factor 1 protein (258-419AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The POU4F1 Antibody (Product code: CSB-PA22749A0Rb) is Non-conjugated. For POU4F1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA22749B0Rb	POU4F1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA22749C0Rb	POU4F1 Antibody, FITC conjugated
Biotin	CSB-PA22749D0Rb	POU4F1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site.; Able to act as transcription factor, cannot regulate the expression of the same subset of genes than isoform 1. Does not have antiapoptotic effect on neuronal cells.

Gene References into Functions

We report a case of a nine-year-old male who presented with facial nerve stimulation four years after cochlear implantation. A dilated internal auditory meatus was revealed. Genetic analysis demonstrated X-linked deafness type 2 (DFNX2) caused by a novel c.769C T nucleotide change in the POU domain, class 3, transcription factor 4 gene PMID: 27863625
Brn3a cooperates with activated RAS/RAF signalling by reducing oncogene-induced senescence in melanocytic tumourigenesis. PMID: 23666755
Data indicate that median methylation levels of BCAN, HOXD1, KCTD8, KLF11, NXPH1, POU4F1, SIM1, and TCF7L1 were >/=30% higher than in normal samples, representing potential biomarkers for tumor diagnosis. PMID: 22930747
PoU4F1 is highly expressed in t(8;21) samples, with AML/ETO appearning to promote some BRN3A expression PMID: 22064348
BRN3A possesses anti-apoptotic property, and considering the above results, it may be regarded as the key component in promoting tumorigenic growth in the uterine cervical cells. PMID: 21928122
Ewing sarcoma induce expression of neuronal markers such as BRN3A showing that the function of those same markers may be restricted or controlled in an sarcoma-dependent manner. PMID: 20348952
Dysregulation of POU4F1 is associated with t(8;21) acute myeloid leukemia. PMID: 20376082
genomic organization of the Brn-3a locus and the mechanisms that control the expression of two different proteins from one genomic locus PMID: 12427558
Measurement of Brn-3a levels in smears can be used to detect a significant proportion of cervical lesions that were missed by Pap smear. PMID: 12893201
These results indicate that Brn-3a could play an important role in the near future in improving cervical cancer screening. PMID: 12911730
oncogenic rearrangement of EWS to produce EWS/Fli-1 may enhance the antiapoptotic effect of Brn-3a and inhibit its ability to promote neuronal differentiation. PMID: 15021903
Hsp27 expression and cell survival are regulated by the POU transcription factor Brn3a PMID: 15272315
Brn-3a has a role in differential regulation of different human papilloma virus variants PMID: 16247485
Upregulation of Brn-3a is associated with prostate cancer PMID: 16276351

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

POU transcription factor family, Class-4 subfamily

Tissue Specificity

Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.

Database Links

HGNC: 9218

OMIM: 601632

KEGG: hsa:5457

STRING: 9606.ENSP00000366413

UniGene: Hs.654522

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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