PUS10 Antibody

Datasheet

Code	CSB-PA565844
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA565844(PUS10 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA565844(PUS10 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse intestinum tenue tissue, Primary antibody: CSB-PA565844(PUS10 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds
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Product Details
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Target Background

Product Details

Uniprot No.

Q3MIT2

Target Names

PUS10

Alternative Names

CCDC139 antibody; Coiled coil domain containing 139 antibody; Coiled coil domain containing protein 139 antibody; Coiled-coil domain-containing protein 139 antibody; DOBI antibody; FLJ32312 antibody; MGC126729 antibody; Pseudouridine synthase 10 antibody; Pseudouridylate synthase 10 antibody; Psi55 synthase antibody; pus10 antibody; PUS10_HUMAN antibody; Putative tRNA pseudouridine synthase Pus10 antibody; tRNA pseudouridine 55 synthase antibody; tRNA pseudouridylate synthase antibody; tRNA uridine isomerase antibody; tRNA-uridine isomerase antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthetic peptide of Human PUS10

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:2000
WB	1:200-1:1000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.

Gene References into Functions

The caspase-3-mediated movement of PUS10 and the release of mitochondrial contents enhancing caspase-3 activity creates a feedback amplification loop for caspase-3 action. Therefore, any defect in the movement or interactions of PUS10 would reduce the TRAIL sensitivity of tumor cells. PMID: 28981101
crystal structure of Pus10 (previously denoted as FLJ32312) at 2.0 A resolution and modeling studies describe protein domains and active sites PMID: 17900615
These data suggest that the cleaved DOBI may acquire a new function, possibly by cooperating with tBid in the mitochondrial event of cell death caused by TRAIL. PMID: 19712588

Subcellular Location

Nucleus. Cytoplasm. Mitochondrion.

Protein Families

Pseudouridine synthase Pus10 family

Database Links

HGNC: 26505

OMIM: 612787

KEGG: hsa:150962

STRING: 9606.ENSP00000326003

UniGene: Hs.368348

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Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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