RAG2 Antibody, HRP conjugated

Code CSB-PA345128LB01HU
Size US$166
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RAG2 Polyclonal antibody
Uniprot No.
Target Names
RAG2
Alternative Names
RAG 2 antibody; RAG-2 antibody; RAG2 antibody; RAG2_HUMAN antibody; Recombination activating gene 2 antibody; V(D)J recombination activating protein 2 antibody; V(D)J recombination-activating protein 2 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human V(D)J recombination-activating protein 2 protein (296-415AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
HRP
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.
Gene References into Functions
  1. Mutation of serine 365 to alanine permits bi-allelic and bi-locus RAG-mediated breaks in the same cell, leading to reciprocal translocations. PMID: 29069605
  2. the ability of the RAG nuclease to minimize the risks of genome disruption by coupling the breakage and repair steps of the V(D)J reaction. This implies that the RAG genes, derived from an ancient transposon, have undergone strong selective pressure to prohibit transposition in favor of promoting controlled DNA end joining in cis by the ubiquitous DNA damage response and DNA repair machineries PMID: 27863852
  3. The show that DNA damage caused by RAG2 activity in pre-B cells was able to downmodulate RAG2 expression and activity, confirming the existence of a negative feedback regulatory mechanism. PMID: 27559048
  4. gene deficiency results in late onset autoimmune hemolytic anemia PMID: 26515615
  5. DNA damage triggers relocalization of RAG2 from the nucleus to centrosomes, suggesting a novel mechanism for modulating cellular responses to double strand breaks in developing lymphocytes. PMID: 25625798
  6. found that Ikaros, a lymphocyte-specific transcription factor, acts as a repressor of NWC promoter--thus identifying a new Ikaros target--but is insufficient for inducing methylation which depends on the antisense transcription driven by RAG-2 promoter PMID: 25198102
  7. The results indicate that the contribution of immune dysregulatory disease due to RAG2 mutations present in the general population may be much higher than previously estimated. PMID: 24472623
  8. Bidirectional activity of the NWC promoter is responsible for RAG-2 transcription in non-lymphoid cells PMID: 22984564
  9. A novel homozygous mutation with different clinical phenotypes: Omenn syndrome and hyper-IgM syndrome PMID: 22841008
  10. analysis of multiorgan metastasis of human HER-2+ breast cancer in Rag2-/-;Il2rg-/- mice and treatment with PI3K inhibitor PMID: 22737248
  11. USF-1 but not USF-2 is strongly enriched at Dbeta2 in chromatin from either Rag2-/- deficient thymocytes or Rag2-/- deficient thymocytes that express a rearranged Tcrb transgene. PMID: 22287717
  12. Analysis of mutations from SCID and Omenn syndrome patients reveals the central role of the Rag2 PHD domain in regulating V(D)J recombination. PMID: 20234091
  13. No sequence changes in RAG2 were found in a series of hematologic leukemias or their cell lines. PMID: 12145704
  14. RAG2 expression and non-functional TCR rearrangements continuously take place in peripheral mature T cells of all activation/differentiation stages PMID: 12355431
  15. intrinsic RAG-2 NLS functions in the nuclear uptake of RAG-2 following its reexpression in cycling cells PMID: 12861017
  16. DNA methylation inhibits the cleavage activity of the RAG1/RAG2 complex by two different mechanisms, depending on the position of the mCpG around the recombination signal sequences PMID: 12897800
  17. Activated mature CD5-positive human tonsil B cells coexpress both RAG2 and RAG1 mRNA and protein, and display DNA cleavage resulting from their recombinase activity. PMID: 15843554
  18. SKP2 mediates ubiquitylation of RAG-2 in vitro and degradation of RAG-2 in vivo. PMID: 15949444
  19. The carboxyl terminus of Rag2 plays a unique role as a direct bridge between chromatin and recombinase during chromosomal V(D)J recombination PMID: 16111638
  20. A knockin murine model carrying the Rag2 R229Q mutation previously described in several patients with Omenn syndrome (OS)supports the notion that impaired immune tolerance and defective immune regulation are involved in the pathophysiology of OS. PMID: 17476358
  21. IL-6 initiates the expression of RAG2 in circulating B cells, and extends those in tonsil B cells. PMID: 17982069
  22. a conserved tryptophan residue (W453) that constitutes a key structural component of the K4me3-binding surface and is essential for RAG2's recognition of H3K4me3 is mutated in patients with immunodeficiency syndromes PMID: 18033247

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Involvement in disease
Combined cellular and humoral immune defects with granulomas (CHIDG); Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive (T(-)B(-)NK(+) SCID); Omenn syndrome (OS)
Subcellular Location
Nucleus.
Protein Families
RAG2 family
Tissue Specificity
Cells of the B- and T-lymphocyte lineages.
Database Links

HGNC: 9832

OMIM: 179616

KEGG: hsa:5897

STRING: 9606.ENSP00000308620

UniGene: Hs.714519

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