REPS1 Antibody

Datasheet

Code	CSB-PA856915LA01HU
Size	US$166
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Image	IHC image of CSB-PA856915LA01HU diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA856915LA01HU diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA856915LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) REPS1 Polyclonal antibody

Uniprot No.

Q96D71

Target Names

REPS1

Alternative Names

RALBP1 antibody; RalBP1 associated Eps domain containing protein 1 antibody; RalBP1 interacting protein 1 antibody; RalBP1-associated Eps domain-containing protein 1 antibody; RalBP1-interacting protein 1 antibody; REPS1 antibody; REPS1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human RalBP1-associated Eps domain-containing protein 1 protein (105-230AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The REPS1 Antibody (Product code: CSB-PA856915LA01HU) is Non-conjugated. For REPS1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA856915LB01HU	REPS1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA856915LC01HU	REPS1 Antibody, FITC conjugated
Biotin	CSB-PA856915LD01HU	REPS1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May coordinate the cellular actions of activated EGF receptors and Ral-GTPases.

Gene References into Functions

Inhibition of RALBP1 by polyclonal antibodies causes increased drug-accumulation and increased cytotoxicity which demonstrate the potential utility of targeting RALBP1 in the treatment of leukemia. PMID: 17143522
RALBP1 and ABCG2 transport doxorubicin differently in lung and breast cancer cell lines PMID: 17273774

Subcellular Location

Membrane, clathrin-coated pit. Note=Colocalize with ITSN1 at the plasma membrane in structures that are most probably clathrin-coated pits.

Tissue Specificity

Widely expressed with highest levels in heart and testis.

Database Links

HGNC: 15578

OMIM: 614825

KEGG: hsa:85021

STRING: 9606.ENSP00000258062

UniGene: Hs.334603

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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