RFX2 Antibody

Code CSB-PA019611LA01HU
Size US$166
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Image
  • IHC image of CSB-PA019611LA01HU diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA019611LA01HU diluted at 1:600 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of MCF-7 cells with CSB-PA019611LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RFX2 Polyclonal antibody
Uniprot No.
Target Names
RFX2
Alternative Names
DNA-binding protein RFX2 antibody; HLA class II regulatory factor RFX2 antibody; regulatory factor X 2 (influences HLA class II expression) antibody; Regulatory factor X 2 antibody; regulatory factor X2 antibody; RFX2 antibody; RFX2_HUMAN antibody; trans acting regulatory factor 2 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human DNA-binding protein RFX2 protein (1-130AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RFX2 Antibody (Product code: CSB-PA019611LA01HU) is Non-conjugated. For RFX2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA019611LB01HU RFX2 Antibody, HRP conjugated ELISA
FITC CSB-PA019611LC01HU RFX2 Antibody, FITC conjugated
Biotin CSB-PA019611LD01HU RFX2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:500-1:1000
IF 1:200-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Transcription factor that acts as a key regulator of spermatogenesis. Acts by regulating expression of genes required for the haploid phase during spermiogenesis, such as genes required for cilium assembly and function. Recognizes and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on promoters. Probably activates transcription of the testis-specific histone gene H1-6.
Gene References into Functions
  1. Rfx2 coordinates multiple, distinct gene expression programs in multi-ciliated epithelial cells, regulating genes that control cell movement, ciliogenesis, and cilia function. PMID: 24424412
  2. We show binding of RFX proteins to an evolutionarily conserved X-box in the ALMS1 proximal promoter and present evidence that these proteins are responsible for ALMS1 transcription during growth arrest PMID: 20381594
  3. novel role for the RFX family of transcription factors as modulators of Ras signalling in epithelial cells. PMID: 15024578
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
RFX family
Database Links

HGNC: 9983

OMIM: 142765

KEGG: hsa:5990

STRING: 9606.ENSP00000306335

UniGene: Hs.465709

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