RPL11 Antibody

Code CSB-PA00065A0Rb
Size US$166
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  • IHC image of CSB-PA00065A0Rb diluted at 1:250 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA00065A0Rb diluted at 1:250 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of MCF-7 cells with CSB-PA00065A0Rb at 1:83, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RPL11 Polyclonal antibody
Uniprot No.
Target Names
RPL11
Alternative Names
60S ribosomal protein L11 antibody; Cell growth inhibiting protein 34 antibody; Cell growth inhibiting protein34 antibody; CLL associated antigen KW 12 antibody; CLL associated antigen KW12 antibody; CLL-associated antigen KW-12 antibody; DBA 7 antibody; DBA7 antibody; GIG 34 antibody; GIG34 antibody; L11 antibody; Ribosomal protein L11 antibody; RL11_HUMAN antibody; RPL 11 antibody; Rpl11 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human 60S ribosomal protein L11 protein (3-178AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RPL11 Antibody (Product code: CSB-PA00065A0Rb) is Non-conjugated. For RPL11 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA00065B0Rb RPL11 Antibody, HRP conjugated ELISA
FITC CSB-PA00065C0Rb RPL11 Antibody, FITC conjugated
Biotin CSB-PA00065D0Rb RPL11 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
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Applications : Immunoblots

Review: Immunoblots for all experimental conditions of the different translationally activated (ITGB3, GJB3, MMP3, CCDC103, TTC30B) or inactivated (RPL11, EIF3G, YBX1) targets in HPP.

By Anonymous

Target Background

Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. Promotes nucleolar location of PML.
Gene References into Functions
  1. PICT-1 is a major nucleolar sensor of the DNA damage repair response and an important upstream regulator of p53 via the RPL11-MDM2-p53 pathway. PMID: 27829214
  2. A novel pathogenic mutation in RPL11 identified in a patient diagnosed with diamond Blackfan anemia as a young adult. PMID: 27667165
  3. RPL splicing variant is associated with Diamond-Blackfan anemia. PMID: 28742285
  4. Simulation of HEY1 Ser-68 phosphorylation prevents its interaction with p53, RPL11 and MDM2 and abolishes HEY1 migration to nucleolar caps upon ribosomal stress. Our findings uncover a novel mechanism for cross-talk between Notch signalling and nucleolar stress PMID: 27129302
  5. Data suggest 5S ribosomal RNA is a direct target of miR-150 and miR-383 in esophageal squamous cell carcinoma (ESCC); overexpression of miR-150 and miR-383 inhibits ESCC cell proliferation in vitro and in vivo and intensifies RPL11/c-Myc interaction. PMID: 26606907
  6. Data indicate that ribosomal protein (L11) promotes the recruitment of microRNA-130a (miR-130a) to oncoprotein c-Myc in response to UV irradiation treatment. PMID: 25544755
  7. Data indicate that ribosomal protein L11 (RPL11)-expressing cells proliferated more rapidly than the ribosomal protein L11 (RPL11)-expressing cells. PMID: 25829192
  8. levels of branched-chain aminotransferase-1 (BCAT1) transcripts are significantly decreased on the polysomes of both RPS19 and RPL11 cells and that translation of BCAT1 protein is especially impaired in cells with small RP gene mutations PMID: 24463277
  9. Findings suggest that PICT1 has a crucial role in gastric cancer progression by regulating the MDM2-TP53 pathway through RPL11. PMID: 24045667
  10. RPL11 mutations led to a dramatic decrease in progenitor cell proliferation and a delayed erythroid differentiation with a marked increase in apoptosis and G0/1 cell cycle arrest with activation of p53. PMID: 22833095
  11. The studies provide insights on how nucleolar stress through L11 and NEDD8 can activate the transcriptional activity of p53. PMID: 22081073
  12. ARF activates p53, at least partly by induction of ribosomal stress, which results in L11 suppression of MDM2 PMID: 22467867
  13. Hydrophilic residues are crucial for ribosomal protein L11 (RPL11) interaction with zinc finger domain of MDM2 and p53 protein activation PMID: 21903592
  14. Results identify a novel regulatory paradigm wherein L11 plays a critical role in controlling c-myc mRNA turnover via recruiting miR-24/miRISC in response to ribosomal stress. PMID: 21807902
  15. Data report that depletion of L37 leads to cell cycle arrest in a MDM2/L11- and p53-dependent manner. PMID: 20935493
  16. L11 suppresses c-Myc-dependent and RNA polymerase III-catalyzed transcription of 5 S rRNA and tRNA genes in response to ribosomal stress, ensuring a tight coordination between c-Myc activity and ribosomal biogenesis. PMID: 20194507
  17. cells survey the maturation of the small and large ribosomal subunits by separate molecular routes, which may merge in an L11-dependent signaling pathway for p53 stabilization. PMID: 20056613
  18. L11 functions as a negative regulator of HDM2 and there might exist in vivo an L11-HDM2-p53 pathway for monitoring ribosomal integrity PMID: 14612427
  19. L11 is not regulated by transcription or protein stability and its level remains relatively constant during serum starvation, which induces translocation of L11 from the nucleolus to the nucleoplasm, where it participates in a complex with HDM2 PMID: 15152193
  20. differentially regulates the levels of ubiquitinated p53 and MDM2 and inhibits the turnover and activity of MDM2 through a post-ubiquitination mechanism PMID: 16803902
  21. These results identify L11 as a feedback inhibitor of c-Myc and suggest a novel role for L11 in regulating c-Myc-enhanced ribosomal biogenesis. PMID: 17599065
  22. Transcription factor YY1 participates in activation transcription of the human ribosomal protein L11 gene PMID: 18389627
  23. RPL11 mutations are associated with abnormal thumbs in Diamond-Blackfan anemia patients. PMID: 19061985
  24. Mutations in RPL11 were identified in two patients from 2 out of 28 families (7.1%). PMID: 19191325
  25. The cell selectively upregulates the translation of mRNAs with a polypyrimidine tract at their 5'-transcriptional start site (5'-TOP mRNAs), including that encoding rpL11, on impairment of 40S ribosome biogenesis. PMID: 19287375
  26. identify NEDD8 as a crucial regulator of L11 RP signalling to p53. A decrease in L11 NEDDylation during nucleolar stress causes relocalization of L11 from the nucleolus to the nucleoplasm. PMID: 19713960

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Involvement in disease
Diamond-Blackfan anemia 7 (DBA7)
Subcellular Location
Nucleus, nucleolus. Cytoplasm.
Protein Families
Universal ribosomal protein uL5 family
Database Links

HGNC: 10301

OMIM: 604175

KEGG: hsa:6135

STRING: 9606.ENSP00000363676

UniGene: Hs.719951

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