SAT2 Antibody

Datasheet

Code	CSB-PA836208LA01HU
Size	US$166
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Image	IHC image of CSB-PA836208LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA836208LA01HU diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-PA836208LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SAT2 Polyclonal antibody

Uniprot No.

Q96F10

Target Names

SAT2

Alternative Names

Amino acid transporter A2 antibody; ATA2 antibody; Diamine acetyltransferase 2 antibody; Diamine N acetyltransferase 2 antibody; Polyamine N acetyltransferase antibody; Polyamine N-acetyltransferase 2 antibody; Protein 40 9 1 antibody; SAT2 antibody; SAT2_HUMAN antibody; SNAT2 antibody; Solute carrier family 38 member 2 antibody; Spermidine/spermine N(1)-acetyltransferase 2 antibody; Spermidine/spermine N1 acetyltransferase 2 antibody; Spermidine/spermine N1 acetyltransferase family member 2 antibody; SSAT2 antibody; System A amino acid transporter 2 antibody; System A transporter 1 antibody; System N amino acid transporter 2 antibody; Thialysine N-epsilon-acetyltransferase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Diamine acetyltransferase 2 protein (1-170AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SAT2 Antibody (Product code: CSB-PA836208LA01HU) is Non-conjugated. For SAT2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA836208LB01HU	SAT2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA836208LC01HU	SAT2 Antibody, FITC conjugated
Biotin	CSB-PA836208LD01HU	SAT2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the N-acetylation of the amino acid thialysine (S-(2-aminoethyl)-L-cysteine), a L-lysine analog with the 4-methylene group substituted with a sulfur. May also catalyze acetylation of polyamines, such as norspermidine, spermidine or spermine. However, ability to acetylate polyamines is weak, suggesting that it does not act as a diamine acetyltransferase in vivo.

Gene References into Functions

Crystallization of this protein and its recombinant form and its complexation with coenzyme A. PMID: 16596569
The expression of three messengers coding for SAT-1, SAT-2 and GalNAcT-1 in human samples of intestinal cancer and some cell lines (breast cancer and melanomas), was evaluated. PMID: 17119850
SSAT2 is an essential component of the ubiquitin ligase complex that regulates hypoxia-inducible factor 1alpha PMID: 17558023
SSAT1, which shares 46% amino acid identity with SSAT2, also binds to HIF-1alpha and promotes its ubiquitination/degradation. However, in contrast to SSAT2, SSAT1 acts by stabilizing the interaction of HIF-1alpha with RACK1 PMID: 17875644
expression of SSAT mediated by Ad-SSAT infection inhibits the growth of colorectal cancer cells PMID: 18949426

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Subcellular Location

Cytoplasm.

Protein Families

Acetyltransferase family

Tissue Specificity

Widely expressed. Under physiological conditions, SSAT2 is expressed at lower level that SSAT1 (SSAT). Many tissues express only SSAT1, several tissues express both SSAT1 and SSAT2, and bone, cervix, ovary and pineal gland expressed only SSAT2.

Database Links

HGNC: 23160

OMIM: 611463

KEGG: hsa:112483

STRING: 9606.ENSP00000269298

UniGene: Hs.10846

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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