SGMS2 Antibody

Datasheet

Code	CSB-PA854159LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat Liver tissue lysate All lanes: SGMS2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42/50 kDa Observed band size: 42/50 kDa IHC image of CSB-PA854159LA01HU diluted at 1:600 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA854159LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SGMS2 Polyclonal antibody

Uniprot No.

Q8NHU3

Target Names

SGMS2

Alternative Names

SGMS2; SMS2; Phosphatidylcholine:ceramide cholinephosphotransferase 2; Sphingomyelin synthase 2

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Phosphatidylcholine:ceramide cholinephosphotransferase 2 protein (1-79AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SGMS2 Antibody (Product code: CSB-PA854159LA01HU) is Non-conjugated. For SGMS2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA854159LB01HU	SGMS2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA854159LC01HU	SGMS2 Antibody, FITC conjugated
Biotin	CSB-PA854159LD01HU	SGMS2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SGMS2 polyclonal antibody was produced by administering the recombinant human phosphatidylcholine: ceramide choline phosphotransferase 2 protein (1-79aa) to a rabbit to elicit an immune response. The resulting rabbit blood was collected and subjected to protein G affinity chromatography to purify the SGMS2 polyclonal antibody, which has a purity of over 95%.

This high-purity antibody has been shown to react with both human and rat samples and has been extensively validated for use in various applications, such as ELISA, WB, IF, and IHC. These applications allow for the detection of the presence and quantity of SGMS2 protein, visualization of its distribution and localization, and identification.

Citations

Expression of Ceramide-Metabolizing Enzymes in the Heart Adipose Tissue of Cardiovascular Disease Patients O Gruzdeva,International journal of molecular sciences,2023

Customer Reviews and Q&A

■ Customer Reviews

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5.0 - 1 reviews

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Applications : Fluorescent Western Blotting

Sample type: cells

Review: Patients with CAD were characterized by higher levels of SGMS2 mRNA in subcutaneous (p = 0.029) and epicardial (p = 0.035) adipocytes.

By Anonymous

Target Background

Function

Sphingomyelin synthase that primarily contributes to sphingomyelin synthesis and homeostasis at the plasma membrane. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction appears to depend on the levels of CER and DAG in the plasma membrane. Does not use free phosphorylcholine or CDP-choline as donors. Can also transfer phosphoethanolamine head group of phosphatidylethanolamine (PE) on to ceramide (CER) to form ceramide phosphoethanolamine (CPE). Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting. To a lesser extent, plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1. Required for normal bone matrix mineralization.

Gene References into Functions

SMS regulates the expression and function of drug transporters P-gp and MRP2. PMID: 27394416
findings suggest that the C-terminal tails of SMSs are involved in homodimer formation, which is required for efficient transport from the ER. PMID: 27927984
PPARdelta activation may be a potential risk of atherosclerosis through enhancing activity of SMS2 PMID: 27278004
F-actin polymerization in the region of HIV-1 membrane fusion was more prominent in Sms2-expressing cells than Sms-deficient cells. PMID: 25231990
SMS1 and SMS2 are capable of regulating TGN-mediated protein trafficking and secretion PMID: 21980337
Data indicate that the increased sphingomyelin mass was due to a rapid and highly specific activation of sphingomyelin synthases SMS1 and SMS2. PMID: 22106271
direct morphological evidence for the pro-atherogenic capabilities of sphingomyelin synthase 2 PMID: 21235823
Data show that SMS2 acting as a bifunctional enzyme with both SM and CPE synthase activity. PMID: 19454763
Data show that sphingomyelin synthases SMS1 and SMS2 are co-expressed in a variety of cell types and function as the key Golgi- and plasma membrane-associated SM synthases in human cervical carcinoma HeLa cells, respectively. PMID: 17449912
Results indicate that both synthase (SMS)1 and 2 contribute to sphingomyelin (SM) de novo synthesis and control SM levels in the cells and on the cell membrane including plasma membrane. PMID: 17616479
SMS2 is a key factor in control of sphingomyelin and diacylglycerol metabolism within the cell, and thus it influences apoptosis. PMID: 17982138
SMS2 regulates subcellular pools of diacylglycerol-binding proteins in the Golgi apparatus. PMID: 18370930
SMS2 (Sphingomyelin synthase 2) physiologically contributes to de novo Sphingomyelin biosynthesis and plasma membrane Sphingomyelin levels PMID: 18566297
Both SMS1 and SMS2 contain two histidines and one aspartic acid which are conserved within the lipid phosphate phosphatase superfamily. Site-directed mutagenesis of these amino acids abolished SMS activity without altering cellular distribution. PMID: 18694848
These results suggested that posttranslational palmitoylation is important for determination of the subcellular localization of SMS2. PMID: 19233134
Sphingomyelin synthase 2 is one of the determinants for plasma and liver sphingomyelin levels in mice. PMID: 19286635

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.

Protein Families

Sphingomyelin synthase family

Tissue Specificity

Brain, heart, kidney, liver, muscle and stomach. Also expressed in a number of cell lines such as carcinoma HeLa cells, hepatoma Hep-G2 cells, and colon carcinoma Caco-2 cells.

Database Links

HGNC: 28395

OMIM: 611574

KEGG: hsa:166929

STRING: 9606.ENSP00000351981

UniGene: Hs.595423

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