SH2D1B Antibody

Code CSB-PA021210LA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA021210LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA021210LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) SH2D1B Polyclonal antibody
Uniprot No.
Target Names
SH2D1B
Alternative Names
SH2D1B antibody; EAT2 antibody; SH2 domain-containing protein 1B antibody; EWS/FLI1-activated transcript 2 antibody; EAT-2 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human SH2 domain-containing protein 1B protein (1-77AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The SH2D1B Antibody (Product code: CSB-PA021210LA01HU) is Non-conjugated. For SH2D1B Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA021210LB01HU SH2D1B Antibody, HRP conjugated ELISA
FITC CSB-PA021210LC01HU SH2D1B Antibody, FITC conjugated
Biotin CSB-PA021210LD01HU SH2D1B Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244. In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation. Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling. In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse. Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation.
Gene References into Functions
  1. When compared with SAP, the other single SH2 domain protein in human, EAT2 shows similar binding energies to unphosphorylated ligands. This is inconsistent to the previous data showing low affinity of EAT2 toward unphosphorylated peptides compared to SAP which shows high affinity PMID: 27586300
  2. Data indicate that Ewing's sarcoma-associated transcript-2 (EAT-2) over-expression increased the anti-tumor activity of NK cells against K562 tumor cell. targets. PMID: 24374770
  3. EAT-2 mediates its effects in natural killer cells by linking SLAM family receptors to phospholipase Cgamma, calcium fluxes, and Erk kinase. PMID: 24687958
Database Links

HGNC: 30416

OMIM: 608510

KEGG: hsa:117157

STRING: 9606.ENSP00000356906

UniGene: Hs.350581

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