SH3BP1 Antibody

Datasheet

Code	CSB-PA23069A0Rb
Size	US$166
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Image	IHC image of CSB-PA23069A0Rb diluted at 1:400 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SH3BP1 Polyclonal antibody

Uniprot No.

Q9Y3L3

Target Names

SH3BP1

Alternative Names

3BP 1 antibody; 3BP-1 antibody; 3BP1_HUMAN antibody; ARHGAP43 antibody; dJ37E16 antibody; FLJ21318 antibody; OTTHUMP00000028981 antibody; SH3 domain binding protein 1 antibody; SH3 domain-binding protein 1 antibody; Sh3bp1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human SH3 domain-binding protein 1 protein (161-266AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SH3BP1 Antibody (Product code: CSB-PA23069A0Rb) is Non-conjugated. For SH3BP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA23069B0Rb	SH3BP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA23069C0Rb	SH3BP1 Antibody, FITC conjugated
Biotin	CSB-PA23069D0Rb	SH3BP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

GTPase activating protein (GAP) which specifically converts GTP-bound Rho-type GTPases including RAC1 and CDC42 in their inactive GDP-bound form. By specifically inactivating RAC1 at the leading edge of migrating cells, it regulates the spatiotemporal organization of cell protrusions which is important for proper cell migration. Also negatively regulates CDC42 in the process of actin remodeling and the formation of epithelial cell junctions. Through its GAP activity toward RAC1 and/or CDC42 plays a specific role in phagocytosis of large particles. Specifically recruited by a PI3 kinase/PI3K-dependent mechanism to sites of large particles engagement, inactivates RAC1 and/or CDC42 allowing the reorganization of the underlying actin cytoskeleton required for engulfment. It also plays a role in angiogenesis and the process of repulsive guidance as part of a semaphorin-plexin signaling pathway. Following the binding of PLXND1 to extracellular SEMA3E it dissociates from PLXND1 and inactivates RAC1, inducing the intracellular reorganization of the actin cytoskeleton and the collapse of cells.

Gene References into Functions

Study demonstrated a high SH3BP1 expression in cervical cancer tissues and correlated with a shorter overall survival. Its expression is even higher in cisplatin resistant cervical neoplasm tissues compared to that of the cisplatin-sensitive ones. Furthermore, SH3BP1 plays an important role in the regulation of cervical cancer cell invasion, migration, and chemoresistance through Rac1/Wav2. PMID: 28786507
Splicing of SH3BP1 and CIN gene loci produces the novel brain specific splice variant BGIN. PMID: 23223568
Epithelial junction formation and morphogenesis require a dual activity complex, containing SH3BP1 and CapZ, that is recruited to sites of active membrane remodeling to guide Cdc42 signaling and cytoskeletal dynamics. PMID: 22891260

Subcellular Location

Cell projection. Cell junction, tight junction. Cell junction, adherens junction. Cell projection, phagocytic cup. Nucleus. Cytoplasm, cytosol.

Database Links

HGNC: 10824

OMIM: 617368

KEGG: hsa:23616

STRING: 9606.ENSP00000350018

UniGene: Hs.601143

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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