SKOR2 Antibody

Datasheet

Code	CSB-PA009018PA01HU
Size	US$166
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Image	IHC image of CSB-PA009018PA01HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA009018PA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of K562 cells with CSB-PA009018PA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SKOR2 Polyclonal antibody

Uniprot No.

Q2VWA4

Target Names

SKOR2

Alternative Names

SKOR2 antibody; CORL2 antibody; FUSSEL18 antibody; SKI family transcriptional corepressor 2 antibody; Functional Smad-suppressing element on chromosome 18 antibody; Fussel-18 antibody; LBX1 corepressor 1-like protein antibody; Ladybird homeobox corepressor 1-like protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence from Human SKI family transcriptional corepressor 2 protein (19-36AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SKOR2 Antibody (Product code: CSB-PA009018PA01HU) is Non-conjugated. For SKOR2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA009018PB01HU	SKOR2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA009018PC01HU	SKOR2 Antibody, FITC conjugated
Biotin	CSB-PA009018PD01HU	SKOR2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Exhibits transcriptional repressor activity. Acts as a TGF-beta antagonist in the nervous system.

Gene References into Functions

Experiments performed using mice, but the observations maybe relevant to other organisms as well. PMID: 18522874
Results verify IRX1, EBF3, SLC5A8, SEPT9, and FUSSEL18 as valid methylation markers in two separate sets of HNSCC specimens; also preliminarily show a trend between HPV16 positivity and target gene hypermethylation of IRX1, EBF3, SLC5A8, and SEPT9. PMID: 20029986

Subcellular Location

Nucleus. Cytoplasm.

Protein Families

SKI family

Tissue Specificity

Expressed in cerebellum, spinal cord and testis. Isoform 2 is present in cerebellum (at protein level).

Database Links

HGNC: 32695

OMIM: 617138

KEGG: hsa:652991

STRING: 9606.ENSP00000414750

UniGene: Hs.585834

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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