SLC46A1 Antibody

Code CSB-PA856995ESR1HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA856995ESR1HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) SLC46A1 Polyclonal antibody
Uniprot No.
Target Names
SLC46A1
Alternative Names
G21 antibody; HCP 1 antibody; HCP1 antibody; Heme carrier protein 1 antibody; MGC9564 antibody; PCFT antibody; PCFT/HCP1 antibody; PCFT_HUMAN antibody; PDE7A antibody; Proton coupled folate transporter antibody; Proton-coupled folate transporter antibody; SLC46A1 antibody; Solute carrier family 46 (folate transporter) member 1 antibody; Solute carrier family 46 member 1 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Proton-coupled folate transporter protein (1-130AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Proton-coupled high-affinity folate and heme transporter that plays an essential role in iron metabolism. Acts as the main importer of heme in the intestine. Imports also heme in the retina and retinal pigment epithelium, in neurons of the hippocampus, and in the renal epithelial cells. Participates therefore in the trafficking of heme and increases intracellular iron content. Mediates also intestinal absorption of folates and their transport from blood to cerebrospinal fluid across the choroid plexus.
Gene References into Functions
  1. Low PCFT expression is associated with treatment response in mesothelioma. PMID: 28945836
  2. our results demonstrate the substantial therapeutic potential of novel 6-substituted pyrrolo[2,3-d]pyrimidine antifolates with dual targeting of PCFT and FRalpha toward Epithelial ovarian cancer that express a range of FRalpha, along with PCFT, as well as cisplatin resistance PMID: 28138029
  3. We found that purified detergent solubilized PCFT was able to bind folic acid, thus indicating a functionally active protein. To assess the oligomeric state negative stain electron microscopy was performed which showed a particle with the size of a PCFT dimer. PMID: 29208467
  4. The 8th transmembrane helix of PCFT plays an important role in defining the aqueous channel and the folate binding pocket. PMID: 28802835
  5. The hydrophobicity of the PCFT W299 residue is important for function suggesting that during the transport cycle this residue interacts with the lipid membrane thereby impacting on the oscillation of the carrier and, indirectly, on the folate binding pocket. PMID: 27251438
  6. we observed nominally significant (but not definitive) associations between SLC46A1 SNPs and NTDs and oral clefts, and this finding warrants follow-up in other populations. PMID: 26789141
  7. A homology model of PCFT, based upon the Escherichia coliglycerol 3-phosphate transporter structure, predicted that PCFT transmembrane domains (TMDs) 1, 2, 7, and 11 form an extracellular gate in the inward-open conformation. PMID: 26884338
  8. We conclude that SLC46A1/PCFT and SLC19A1/RFC-1 are associated with DFS of patients with colorectal cancer and hypothesize that poor response to 5-fluorouracil plus leucovorin therapy in some patients may be linked to low expression of these genes. PMID: 26193446
  9. Report sustained inhibition of PCFT by myricetin. PMID: 25801697
  10. this is the first study to simultaneously evaluate both DNA methylation and protein expression of all three folate transporter genes, FOLR1, PCFT, and RFC1, in colorectal cancer. PMID: 25697897
  11. results imply that TMDs 3 and 6 provide critical interfaces for formation of hPCFT oligomers, which might be facilitated by the GXXXG motifs in TMD2 and TMD4. PMID: 25877470
  12. the RFC1 G80A polymorphism does not seem to be a good marker of MTX-related toxicity in pediatric ALL. PMID: 24597986
  13. The current study addresses the role of Tyr residues in SLC46A1 function; and identifies four residues that enhance the affinity of the carrier for its substrates and decrease the rate at which the carrier oscillates between its conformational states. PMID: 25608532
  14. SLC46A1 genotype may help to identify patients with increased risk of methotrexate-related toxicity. PMID: 25124723
  15. Data indicate that 28/33 cysteine-less proton-coupled folate transporter (PCFT) mutant HeLa cells were active for [(3)H]methotrexate uptake. PMID: 25053408
  16. The molecular bases for methotrexate resistance associated with loss of SLC19A1 transport and for hereditary folate malabsorption, attributable to mutant SLC46A1, were determned (review). PMID: 24396145
  17. SLC46A1 SNP had a statistically significant association with HDL plasma levels. PMID: 23656756
  18. At weakly acidic pH (6.5), bisulfite and nitrite exhibited much stronger inhibition of PCFT-mediated transport. PMID: 23609145
  19. Two loss-of-function mutations in the fourth transmembrane domain of proton-coupled folate transporter (PCFT) play a role in hereditary folate malabsorption in subjects with this disorder. PMID: 23552283
  20. These results suggest that the activity of PCFT promoter is basically induced by KLF4 and the gradiented expression profile of PCFT may be at least in part accounted for by those of HNF4alpha, CDX2 and C/EBPalpha. PMID: 23313509
  21. The monomeric state of proton-coupled folate transporter is the functional state, substrate translocation does not depend on homo-oligomerization. PMID: 23601781
  22. PCFT is abundantly expressed in human tumors and is active at pHs characterizing the tumor microenvironment PMID: 22954694
  23. Loss of intrinsic Gly338Cys-PCFT function is due solely to impaired oscillation of the carrier between its conformational states. PMID: 22843796
  24. The role of GXXG motif is consistent with a molecular structural model in which this motif and Ile188 are accessible to the PCFT aqueous translocation pathway. PMID: 22785121
  25. HCP1 is involved in low-affinity heme-Fe uptake by Caco-2 cells. PMID: 22496243
  26. the P425R-PCFT mutation produces a conformational change that fully preserves pemetrexed binding but markedly impairs binding of methotrexate and other folates to the carrier. PMID: 22345511
  27. Identification and functional impact of homo-oligomers of the human proton-coupled folate transporter. PMID: 22179615
  28. PCFT 928GG genotype had significantly higher plasma Hcy concentrations compared with carriers of the A allele. PMID: 21338559
  29. These findings are consistent with a common mutation in the PCFT gene causing hereditary folate malabsorption that has disseminated to Puerto Ricans who have migrated to mainland United States PMID: 21489556
  30. novel mutations are described in three subjects with folate malabsorption: A335D/N68Kfs (c.1004C>A/c.204-205delCC), compound heterozygous mutations, and two homozygous PCFT mutations, G338R (c.1012G>C) and E9Gfs (c.17-18insC). PMID: 21333572
  31. Random mutagenesis of the proton-coupled folate transporter (SLC46A1), clustering of mutations, and the bases for associated losses of function. PMID: 21602279
  32. Data show that the loss of activity of mutant PCFTs was shown to be due to impaired protein stability and expression. PMID: 21256110
  33. The report on a Turkish family with 2 children with HFM who have a novel homozygous frameshift mutation (c.204 205delCC) in the PCFT gene. PMID: 20795774
  34. D156 plays a critical role in PCFT protein stability, and D109, located in the first intracellular loop between the second and third transmembrane domains, is absolutely required for PCFT function. PMID: 20805364
  35. the obligatory intestinal folate transporter PCFT (SLC46A1) is regulated by nuclear respiratory factor 1 PMID: 20724482
  36. Data suggest that mutation of the R376 residue of SLC46A1 to Gln impairs proton binding which modulates the folate-binding pocket and depresses the rate of conformational alteration of the carrier. PMID: 20686069
  37. analyses establish a PCFT secondary structure of 12 transmembrane domains with the N- and C- termini directed to the cytoplasm PMID: 20225891
  38. Direct sequencing of PCFT revealed a novel homozygous frameshift mutation (c.194dupG) at a mononucleotide repeat in exon 1 predicted to result in a truncated protein (p.Cys66LeufsX99) causing hereditary folate malabsorptionl PMID: 20005757
  39. Using immunohistochemistry, PCFT was localized to microvillous plasma membrane of syncytiotrophoblasts during first trimester and at term. PMID: 20036773
  40. hPCFT transporter activity can be modulated by many drugs in clinical use, and expression of this transporter in the gastrointestinal tract is higher in the duodenum than more distal sites (duodenum > ileum > colon) PMID: 19762432
  41. A loss-of-function mutation in PCFT/HCP1 is the molecular basis for hereditary folate malabsorption in a family with this disease. PMID: 17129779
  42. HCP1 expression in different cells implies a functional role in tissues other than the duodenum PMID: 17156779
  43. Our data revealed low-level wider expression of human HCP1 transcript in multiple tissues suggesting that it is responsible for heme transport in the body, not the intestine alone. PMID: 17335806
  44. Mutations in the PCFT gene is associated with malabsorption syndromes PMID: 17446347
  45. Data suggest that hPCFT/HCP1 is responsible for the intestinal absorption of folate and also methotrexate. PMID: 17475902
  46. HCP-1 expression in human macrophages is downregulated by TLR agonists & IFN-gamma, upregulated by dexamethasone. In early endosomes, it colocalizes with endocytosed Hb-haptoglobin (Hp) complexes, taken up by the CD163 scavenger receptor pathway. PMID: 17947394
  47. Present information regarding structure-function and membrane targeting of the hPCFT polypeptide, as well as the mechanisms that control its steady-state expression in polarized cells. PMID: 18003745
  48. Intestinal folate uptake process undergoes differentiation-dependent regulation and that this regulation is mediated via changes in the level of expression of both the RFC and PCFT. PMID: 18650265
  49. HFM was shown to be due to loss-of-function mutations in a proton-coupled folate transporter (PCFT),[3] and [4] allowing the possibility of rapid diagnosis and correlations among specific mutations, clinical manifestations, and outcome. PMID: 18718264
  50. These findings constitute the first demonstration of the dominant epigenetic silencing of the PCFT gene in leukemia cells. PMID: 18817749

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Involvement in disease
Hereditary folate malabsorption (HFM)
Subcellular Location
Apical cell membrane; Multi-pass membrane protein. Cytoplasm.
Protein Families
Major facilitator superfamily, SLC46A family
Tissue Specificity
Expressed in kidney, liver, placenta, small intestine, spleen, retina and retinal pigment epithelium. Lower levels found in colon and testis. Very low levels in brain, lung, stomach, heart and muscle. In intestine, expressed in duodenum with lower levels
Database Links

HGNC: 30521

OMIM: 229050

KEGG: hsa:113235

STRING: 9606.ENSP00000395653

UniGene: Hs.446689

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