SPAG8 Antibody

Datasheet

Code	CSB-PA858730LA01HU
Size	US$166
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Image	Immunofluorescence staining of PC-3 cells with CSB-PA858730LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SPAG8 Polyclonal antibody

Uniprot No.

Q99932

Target Names

SPAG8

Alternative Names

BS 84 antibody; CILD28 antibody; HSD 1 antibody; HSD-1 antibody; HSD1 antibody; hSMP1 antibody; MGC26201 antibody; MH SPAG8 antibody; OTTHUMP00000021352 antibody; OTTMUSP00000032209 antibody; SMP-1 antibody; SMP1 antibody; SPAG3 antibody; SPAG8 antibody; SPAG8_HUMAN antibody; Sperm associated antigen 8 antibody; Sperm associated antigen 8 like protein antibody; Sperm membrane protein 1 antibody; Sperm membrane protein BS-84 antibody; Sperm-associated antigen 8 antibody; Testicular tissue protein Li 177 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Sperm-associated antigen 8 protein (236-409AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SPAG8 Antibody (Product code: CSB-PA858730LA01HU) is Non-conjugated. For SPAG8 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA858730LB01HU	SPAG8 Antibody, HRP conjugated	ELISA
FITC	CSB-PA858730LC01HU	SPAG8 Antibody, FITC conjugated
Biotin	CSB-PA858730LD01HU	SPAG8 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau. Involved in the acrosome reaction and in binding of sperm to the zona pellucida. Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis. May play a role in fertility and microtubule formation through interaction with RANBP9.

Gene References into Functions

Results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis. PMID: 20488182
The antibody of this recombinant protein inhibits the mouse acrosome reaction and prmits mouse sperm-zona pellucida binding. PMID: 17187156
Sperm associated antigen 8 might be involved in the regulation of cell cycle by changing the phosphorylation level of Tyr15 on CDC2 Protein Kinase. PMID: 19548270

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic vesicle, secretory vesicle, acrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, spindle.

Protein Families

SPAG8 family

Tissue Specificity

Expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine.

Database Links

HGNC: 14105

OMIM: 605731

KEGG: hsa:26206

STRING: 9606.ENSP00000340982

UniGene: Hs.256747

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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