Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SRPK2 Polyclonal antibody

Uniprot No.

P78362

Target Names

SRPK2

Alternative Names

Human serine kinase SRPK2 mRNA, complete cds antibody; Serine kinase SRPK2 antibody; Serine/arginine rich protein specific kinase 2 antibody; Serine/arginine-rich protein-specific kinase 2 antibody; Serine/threonine protein kinase SRPK2 antibody; SFRS protein kinase 2 antibody; SFRSK2 antibody; SR protein specific kinase 2 antibody; SR-protein-specific kinase 2 antibody; SRPK2 antibody; SRPK2_HUMAN antibody; SRSF protein kinase 2 C-terminal antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human SRSF protein kinase 2 protein (471-688AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

HRP

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Probably by phosphorylating DDX23, leads to the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.

Gene References into Functions

SRPK2 may play an important role in the progression and metastasis of prostate cancer. PMID: 29587239
pausing of RNA polymerase II (RNA Pol II) initiates a signaling cascade whereby the serine/arginine protein kinase 2 (SRPK2) phosphorylates the DDX23 helicase, culminating in the suppression of R-loops. PMID: 28076779
ten candidate variants were prioritised. Of these, SRPK2 (c.2044C>T[p.Arg682Trp]) and NOTCH1 (c.3835C>T[p.Arg1279Cys]), co-segregated with disease in the family; however, previous functional analyses on SRPK2 make this an unlikely candidate. Functional analyses in the variant (c.3835C>T[p.Arg1279Cys]) of the known CHD gene NOTCH1 demonstrated a non-significant decrease in signalling activity. PMID: 27989580
Delta-secretase phosphorylation by SRPK2 plays a critical role in aggravating Alzheimer disease pathogenesis. PMID: 28826672
In this study, it was found that the expression of SRPK2 was up-regulated in the clinical colon cancer samples. Overexpression of SRPK2 promoted the growth and migration of colon cancer cells, while knocking down the expression of SRPK2 inhibited the growth, migration and tumorigenecity of colon cancer cells. PMID: 27041240
Our results indicate that phosphorylation of SRPK2 plays a crucial role in the regulation of splicing process in head and neck squamous cell carcinoma (HNSCC), and that splicing kinases can be developed as a new class of therapeutic target in HNSCC. PMID: 26853621
A conserved electronegative docking groove in SRPK2 is responsible for substrate binding. PMID: 24444330
the BLRF2 RS motif is phosphorylated by SRPK2 and is important for viral replication. PMID: 23326445
Short-term exercise resulted in a significant increase of mRNA expression of genes encoding proteins involved in the formation of precatalytic splisosome: SRPK2. PMID: 19902070
SRPK2 knock down results in hypophosphorylation of the arginine-serine (RS) domain-containing human PRP28 protein (PRP28, also known as DDX23), and destabilizes PRP28 association with the tri-snRNP. PMID: 18425142
overexpression of SRPK2 is associated with phosphorylating acinus and regulating its stimulatory effects on cyclin A1 expression, contributing to leukemia cell proliferation PMID: 18559500
Interaction of Akt-phosphorylated SRPK2 with 14-3-3 mediates cell cycle and cell death in neurons. PMID: 19592491