STXBP5 Antibody

Datasheet

Code	CSB-PA722568LA01HU
Size	US$166
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Image	IHC image of CSB-PA722568LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA722568LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) STXBP5 Polyclonal antibody

Uniprot No.

Q5T5C0

Target Names

STXBP5

Alternative Names

STXBP5 antibody; LLGL3 antibody; Syntaxin-binding protein 5 antibody; Lethal(2) giant larvae protein homolog 3 antibody; Tomosyn-1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Syntaxin-binding protein 5 protein (515-610AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The STXBP5 Antibody (Product code: CSB-PA722568LA01HU) is Non-conjugated. For STXBP5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA722568LB01HU	STXBP5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA722568LC01HU	STXBP5 Antibody, FITC conjugated
Biotin	CSB-PA722568LD01HU	STXBP5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release. Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane. Competes with STXBP1 for STX1 binding.

Gene References into Functions

Glucose-dependent de-SUMOylation of tomosyn1 at K298 releases syntaxin1A and controls the amplification of exocytosis in concert with a recently-identified tomosyn1-interacting partner; the Ca(2+)-binding protein secretagogin, which dissociates from tomosyn1 in response to Ca(2+)-raising stimuli and is required for insulin granule trafficking and exocytosis downstream of Ca(2+) influx. PMID: 28325894
Using CRISPR/Cas9 genome editing, identified a human nonsynonymous SNP rs1039084 in the STXBP5 locus as a causal variant for a decreased thrombotic phenotype. PMID: 28062498
Genetic variations in STXBP5 and CLEC4M are associated with VWF level variation in type 1, but not in type 2 von Willebrand disease. PMID: 25832887
STXBP5 is required for normal arterial hemostasis, due to its contributions to platelet granule cargo packaging and secretion PMID: 25244094
STXBP5 inhibits endothelial exocytosis and promotes platelet secretion PMID: 25244095
Identify 3 loci associated with circulating tPA levels, the PLAT region, STXBP5, and STX2. Functional studies implicate a novel role for STXBP5 and STX2 in regulating tPA release. PMID: 24578379
Genetic variation in STXBP5 is associated with bleeding phenotype in female type 1 von Willebrand Disease patients. PMID: 22792389
Genetic variation in STXBP5 gene is associated with venous thrombosis. PMID: 21163921
multiple domains outside the R-SNARE of tomosyn are critical to the efficacy of inhibition by tomosyn on exocytotic secretion PMID: 21330375
Genetic variability in STXBP5 and STX2 affects both VWF concentration and activity in young individuals with premature arterial thrombosis. PMID: 21156930
Characterization of a related rat protein PMID: 9620695
Characterization of a related rat gene PMID: 10066450

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cell junction, synapse.

Protein Families

WD repeat L(2)GL family

Database Links

HGNC: 19665

OMIM: 604586

KEGG: hsa:134957

STRING: 9606.ENSP00000321826

UniGene: Hs.736439

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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