TCAF2 Antibody

Datasheet

Code	CSB-PA008015LA01HU
Size	US$166
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Image	IHC image of CSB-PA008015LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA008015LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA008015LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TCAF2 Polyclonal antibody

Uniprot No.

A6NFQ2

Target Names

TCAF2

Alternative Names

TCAF2 antibody; FAM115C antibody; FAM139A antibody; TRPM8 channel-associated factor 2 antibody; TRP channel-associated factor 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human TRPM8 channel-associated factor 2 protein (472-590AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TCAF2 Antibody (Product code: CSB-PA008015LA01HU) is Non-conjugated. For TCAF2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA008015LB01HU	TCAF2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA008015LC01HU	TCAF2 Antibody, FITC conjugated
Biotin	CSB-PA008015LD01HU	TCAF2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Negatively regulates the plasma membrane cation channel TRPM8 activity. Involved in the recruitment of TRPM8 to the cell surface. Promotes prostate cancer cell migration stimulation in a TRPM8-dependent manner.

Gene References into Functions

Report novel TRP channel-associated factors that modulate TRPM8 activity. PMID: 25559186

Subcellular Location

[Isoform 2]: Cell membrane.

Protein Families

TCAF family

Tissue Specificity

Isoform 2 is expressed in the prostate and in cancerous prostate samples.

Database Links

HGNC: 26878

OMIM: 616252

KEGG: hsa:285966

UniGene: Hs.743565

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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