TCHP Antibody

Datasheet

Code	CSB-PA874804LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA874804LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA874804LA01HU at dilution of 1:100 Immunofluorescence staining of MCF-7 cells with CSB-PA874804LA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TCHP Polyclonal antibody

Uniprot No.

Q9BT92

Target Names

TCHP

Alternative Names

TCHP antibody; Trichoplein keratin filament-binding protein antibody; Protein TCHP antibody; Mitochondrial protein with oncostatic activity antibody; Mitostatin antibody; Tumor suppressor protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Trichoplein keratin filament-binding protein (1-498AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TCHP Antibody (Product code: CSB-PA874804LA01HU) is Non-conjugated. For TCHP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA874804LB01HU	TCHP Antibody, HRP conjugated	ELISA
FITC	CSB-PA874804LC01HU	TCHP Antibody, FITC conjugated
Biotin	CSB-PA874804LD01HU	TCHP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells. Is a negative regulator of ciliogenesis.

Gene References into Functions

Ndel1 acts as a novel upstream regulator of the trichoplein-Aurora A pathway to inhibit primary cilia assembly. PMID: 26880200
These findings underscore the complexity of PGC-1alpha-mediated mitochondrial homeostasis and establish mitostatin as a key regulator of tumor cell mitophagy and angiostasis. PMID: 24403067
Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. PMID: 21325031
Trichoplein/mitostatin is a new regulator of mitochondria-endoplasmic reticulum juxtaposition. PMID: 20930847
MITOSTATIN was found within a 3.2-kb transcript for an approximately 62 kDa mitochondrial protein with tumor suppressor activity. It inhibits cell growth, is proapoptotic and downregulates Hsp27. PMID: 18931701
trichoplein is a keratin 8/18-binding protein that may be involved in the organization of the apical network of keratin filaments and desmosomes in simple epithelial cells PMID: 15731013

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Subcellular Location

Cytoplasm, cytoskeleton. Cytoplasm. Cell membrane. Mitochondrion. Cell junction, desmosome.

Protein Families

TCHP family

Tissue Specificity

Expressed at high levels in normal urothelial and breast epithelial cells. Also expressed in the smooth muscle and endothelial cells. Reduced expression seen in advanced bladder and breast carcinomas (at protein level). Ubiquitous. Expressed at highest le

Database Links

HGNC: 28135

OMIM: 612654

KEGG: hsa:84260

STRING: 9606.ENSP00000324404

UniGene: Hs.410924

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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