TMEM127 Antibody

Datasheet
Code CSB-PA023694LA01HU
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Size US$299
Uniprot No. O75204
Image
  • IHC image of CSB-PA023694LA01HU diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of A549 cells with CSB-PA023694LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Transmembrane protein 127 protein (1-95AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200
Relevance Controls cell proliferation acting as a negative regulator of TOR signaling pathway mediated by mTORC1. May act as a tumor suppressor.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Transmembrane protein 127, TMEM127
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Cell Biology
Target Names TMEM127
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Controls cell proliferation acting as a negative regulator of TOR signaling pathway mediated by mTORC1. May act as a tumor suppressor.
Involvement in disease Pheochromocytoma (PCC)
Subcellular Location Cell membrane, Multi-pass membrane protein, Cytoplasm
Protein Families TMEM127 family
Tissue Specificity Widely expressed.
Database Links

HGNC: 26038

OMIM: 171300

KEGG: hsa:55654

STRING: 9606.ENSP00000258439

UniGene: Hs.164303

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