TNP2 Antibody

Datasheet
Code CSB-PA024019LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q05952
Image
  • IHC image of CSB-PA024019LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA024019LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Nuclear transition protein 2 protein (1-138AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200
Relevance Plays a key role in the replacement of histones to protamine in the elongating spermatids of mammals. In condensing spermatids, loaded onto the nucleosomes, where it promotes the recruitment and processing of protamines, which are responsible for histone eviction.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Nuclear transition protein 2 (TP-2) (TP2), TNP2
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names TNP2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Plays a key role in the replacement of histones to protamine in the elongating spermatids of mammals. In condensing spermatids, loaded onto the nucleosomes, where it promotes the recruitment and processing of protamines, which are responsible for histone eviction.
Subcellular Location Nucleus, Chromosome
Protein Families Nuclear transition protein 2 family
Tissue Specificity Expressed by spermatids (at protein level).
Database Links

HGNC: 11952

OMIM: 190232

KEGG: hsa:7142

STRING: 9606.ENSP00000325738

UniGene: Hs.513349

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