TOP1MT Antibody, FITC conjugated

Datasheet
Code CSB-PA853372LC01HU
Size US$299
Uniprot No. Q969P6
Protocols ELISA Protocol
Immunogen Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA
Relevance Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Form Liquid
Conjugate FITC
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias DNA topoisomerase I, mitochondrial (TOP1mt) (EC 5.99.1.2), TOP1MT
Immunogen Species Homo sapiens (Human)
Research Area Epigenetics and Nuclear Signaling
Target Names TOP1MT
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Subcellular Location Mitochondrion
Protein Families Type IB topoisomerase family
Tissue Specificity Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.
Database Links

HGNC: 29787

OMIM: 606387

KEGG: hsa:116447

STRING: 9606.ENSP00000328835

UniGene: Hs.528574

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