TRIM5 Antibody

Code CSB-PA874861LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, Mouse heart tissue
    All lanes: TRIM5 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 57, 47, 41, 38, 32, 30 kDa
    Observed band size: 70 kDa

  • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA874861LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA874861LA01HU at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) TRIM5 Polyclonal antibody
Uniprot No. Q9C035
Target Names TRIM5
Alternative Names RING finger protein 88 antibody; RNF88 antibody; TRIM5 antibody; TRIM5_HUMAN antibody; TRIM5alpha antibody; Tripartite motif containing 5 antibody; tripartite motif protein TRIM5 antibody; Tripartite motif-containing protein 5 antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Tripartite motif-containing protein 5 protein (1-493AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of Ca2+ Signaling in the Determination of Skeletal Muscle Development Potential.. K Qiu,Cells,2020

Applications: Western Blot
Sample type: Muscle Myo-lineage cells
Review: In order to verify the reliability of proteomics data, 7 DEPs were randomly selected for Western blot analysis. As shownin FigureS1, there lativea bundance sof selected proteins between Myo-L and Myo-Y determined by Western blot were highly consistent with the data of TMT analysis.
PMID: 32331484

Target Data

Function Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV)
Gene References into Functions
  1. study demonstrates the feasibility of editing the TRIM5 gene in human cells and identifies the main challenges to be addressed in order to use this approach to confer protection from HIV-1 PMID: 29373607
  2. genetic polymorphism is associated with susceptibility to HIV infections in Brazil PMID: 27388872
  3. TRIM5 and TRIM22 single nucleotide polymorphisms are associated to increased odds of significant liver fibrosis and sustained virological response after pegIFNalpha/RBV therapy in human immunodeficiency virus/hepatitis C virus coinfected patients. PMID: 27590274
  4. TRIM5alpha polymorphisms were associated with proviral loads, indicating that TRIM5alpha could be implicated in HTLV-1 replication. PMID: 28420387
  5. TRIM5alpha potently restricts HIV-1 infection of Langerhans cells but not of subepithelial DC-SIGN+ dendritic cells PMID: 27919079
  6. Collectively, these results are consistent with observations that the turnover of TRIM5alpha proteins is sensitive to autophagy inhibition; however, the data presented here do not support observations that the inhibition of autophagy abrogates retroviral restriction by TRIM5 proteins. PMID: 26764007
  7. This meta-analysis indicates that TRIM5alpha H43Y polymorphism is associated with a decreased risk of HIV-1 infection in the homozygote comparison and recessive model. PMID: 26398573
  8. These results support the relevance of R332G-R335G and other mutants of TRIM5alphahu as candidate effectors for HIV-1 gene therapy. PMID: 26076730
  9. higher-order oligomerization of TRIM5alpha, which is promoted by the interaction with the retroviral capsid, enhances the E3 Ub ligase activity of TRIM5alpha and contributes to its antiretroviral function. PMID: 26212332
  10. co-immunoprecipitation experiments demonstrate that IE1CORE binds via the coiled-coil domain to PML and also interacts with TRIM5alpha PMID: 25412268
  11. Data suggest that due to its lack of stability and inability to accumulate in pronounced cytoplasmic bodies likely due to its high self-ubiquitination activity, huTRIM5alpha was unable to block HIV-1 infection. PMID: 24662946
  12. TRIM5alpha variations influence transduction efficiency with lentiviral vectors in both human and rhesus CD34(+) cells in vitro and in vivo. PMID: 24153115
  13. TRIMs interacts with ULK1 and Beclin 1 and regulates autophagy. PMID: 25127057
  14. TRIM5 acts as a selective autophagy receptor. Based on direct sequence-specific recognition, TRIM5 delivered its cognate cytosolic target, a viral capsid protein, for autophagic degradation. Thus, our study establishes that TRIMs can function both as regulators of autophagy and as autophagic cargo receptors, and reveals a basis for selective autophagy in mammalian cells. PMID: 25127057
  15. TRIM5alpha and TRIM22 have differential transcriptional regulation and distinct anti-HIV roles according to infection phase. PMID: 24478420
  16. In conclusion, association with microtubules and the translocation activity of dynein motor complexes are required to achieve efficient retrovirus restriction by TRIM5alpha. PMID: 24600008
  17. Data report that markers in two TRIMs, TRIM5 and TRIM22 and a marker in BST2, associated statistically with the risk of getting MS. PMID: 24066097
  18. Our data indicate that although the RhTRIMe7-CypA isoform does not appear to restrict HIV-1, it may act as a negative modulator of TRIM family proteins, presumably by competitive inhibition PMID: 24613845
  19. structural changes exerted on HIV-1 capsid (CA) assembly by TRIM5alpha binding PMID: 24158810
  20. Assisted evolution enables HIV-1 to overcome a high TRIM5alpha-imposed genetic barrier to rhesus macaque tropism. PMID: 24086139
  21. recruitment to the plasma membrane plays role in restriction of retroviral infection PMID: 23548691
  22. These study supports a model in which localized binding of TRIM5 to the retrovirus capsid nucleates rapid polymerization of a TRIM5 lattice on the capsid surface. PMID: 23785198
  23. HIV-2 capsid sequences expressed high levels of susceptibility to hTRIM5alpha. PMID: 23647667
  24. results show how TRIM5alpha affects various retroviral core components and indicate that proteasomes are required for TRIM5alpha-induced core disruption but not for TRIM5alpha-induced restriction PMID: 23505372
  25. These data suggest that HIV-1 escapes restriction by TRIM5alpha through the selective disruption of CypA-dependent, TRIM5alpha-mediated inhibition of nuclear import. PMID: 23448277
  26. Blocking the onset or delaying reverse transcription does not increase HIV-1 sensitivity to TRIM5alpha, indicating that the recognition of the capsids by human TRIM5alpha is completed rapidly, following entry into the cytoplasm. PMID: 23320071
  27. amino acid substitution G249D associated with increased susceptibility to HIV-1 infection PMID: 23379364
  28. The recognition of the entire capsid surface is a general strategy for TRIM5alpha to restrict murine leukemia viruses. PMID: 23536686
  29. Increased sensitivity to TRIM5alpha was observed for some capsid variants, suggesting that minor residues are selected against in human populations. PMID: 23601783
  30. TRIM5 is a restriction factor that blocks retrovirus infection soon after the virion core enters the cell cytoplasm. Restriction activity is targeted to the virion core via recognition of the capsid protein lattice that encases the viral genomic RNA.[Review] PMID: 22482711
  31. Findings have defined a novel function for TRIM5 as a pattern recognition receptor in innate immune recognition and provided valuable mechanistic insight into its role as a retroviral restriction factor. PMID: 21866272
  32. Proteasomal subunits are present in rhTRIM5alpha assemblies containing HIV-1 virions. PMID: 22078707
  33. Determinants of the higher order association of the restriction factor TRIM5alpha and other tripartite motif (TRIM) proteins. PMID: 21680743
  34. TRIM5alpha "cages" the HIV-1 core by forming an hexagonal array on the surface of the viral capsid. PMID: 21994740
  35. findings suggest that Gag cytotoxic T lymphocyte mutations may influence HIV-1 replication by modifying both viral infectivity and sensitivity to TRIM5alpha PMID: 21917976
  36. study concludes that interactions involving much, if not all, of the surface of the murine leukemia virus capsid protein are vital for TRIM5alpha binding PMID: 21483490
  37. The authors discovered the ability of human and rhesus TRIM5alpha to shuttle into and out of the nucleus. PMID: 21575157
  38. review of the structure and roles of TRIM5alpha protein, the interaction between Cyp cyclophilin A and TRIM5alpha, as well as gene therapy strategies associated with TRIM5alpha to inhibit HIV-1 infection[review] PMID: 21568899
  39. knockdown of TRIM5iota increases TRIM5alpha activity in human U373-X4 cells, indicating that physiological levels of expression of truncated TRIM5 isoforms in human cells can reduce the activity of TRIM5 PMID: 21632761
  40. The SUMO-1-mediated block of murine leukemia virus is mediated by human TRIM5alpha. CA mutations altering the SUMO conjugation sites reduce TRIM5alpha restriction. PMID: 21490953
  41. the N-terminal region of TRIM5alphaag and TRIM5alphacy are essential for the late restriction activity, while the C-terminal region of TRIM5alphacy negatively regulates the late restriction activity against HIV-1 PMID: 21264255
  42. the retroviral restriction factor TRIM5 has two additional activities that are linked to restriction: it constitutively promotes innate immune signalling and it acts as a pattern recognition receptor specific for the retrovirus capsid lattice PMID: 21512573
  43. retroviruses have evolved similar mechanisms to escape TRIM5alpha restriction via the interference of structurally homologous determinants in the viral capsid. PMID: 21169362
  44. The authors concluded that H43Y might account for the HIV-1 resistance due to TRIM5alpha gene in Chinese intravenous drug users. PMID: 21107267
  45. Innate immunity mediated by envelope of murine leukemia virus in human cells is TRIM5alpha independent. PMID: 20929586
  46. TRIM5alpha proteins have evolved to restrict a range of different retroviruses by assembling a deformable hexagonal scaffold that positions the capsid-binding domains to match the symmetry and spacing of the capsid surface lattice PMID: 21187419
  47. Both common and rare variants of TRIM5alpha are associated with the susceptibility to HIV-1 infection in Japanese and Indian subjects. PMID: 19710594
  48. The ability of human Trim5alpha to regulate TAB2 levels, to activate NF-kappaB, and to recognize retroviral capsids are genetically separable. PMID: 21035162
  49. These findings suggest that human TRIM5alpha is an intrinsic immunity factor against HIV-1 infection. PMID: 20493962
  50. Cytoplasmic body component TRIM5{alpha} requires lipid-enriched microdomains for efficient HIV-1 restriction. PMID: 20810659
  51. G116E substitution in the capsid protein of the HIV-1 recombinant NL-4/5S6/7SvifS virus rendered the virus resistant to cynomolgus monkey, but not human TRIM5 alpha-mediated restriction. PMID: 20609213
  52. HIV-1 displays virus-specific differences in its sensitivity to human TRIM5alpha and in its sensitivity to different TRIM5alpha alleles. PMID: 20702630
  53. REVIEW: current knowledge on the anti-retroviral effects of TRIM5 alpha and TRIM22 PMID: 19943174
  54. Our data suggest that MDM2 is a general but nonessential modulator of TRIM5alpha-mediated antiretroviral functions. PMID: 20619429
  55. Sequestosome-1 associates with and enhance the expression of retroviral restriction factor TRIM5alpha. PMID: 20357094
  56. TRIM5 alpha recognises the multimerised capsid (viral core) of an incoming virus by its alpha-isoform specific SPRY domain and is believed to be involved in innate immunity to control retroviral infection. PMID: 20049904
  57. These results suggest that Hsp70 plays critical roles in the cellular management against the TRIM5alpha-induced cellular insults. PMID: 20053985
  58. Results describe the action of a human HIV-1 inhibitor modeled after AoT5Cyp, constructued by fusing human CypA to human TRIM5. PMID: 19741300
  59. Data show significant associations between SNPs in RARA, RARB, TOP2B and RARG, RXRA, TLR3, TRIM5 and RIG-I genes and rubella virus-specific cytokine immune responses. PMID: 19902255
  60. increased representation of the minor allele of the 5'UTR SNP (rs3824949) in the antiretroviral TRIM5(tripartite motif protein5) gene was associated with an allele dose-related increase in rubella antibody. PMID: 20001730
  61. TRIM5delta colocalized with BTBD1/2 and appeared to serve as a scaffold for the assembly of endogenous BTBD1/2 proteins PMID: 12878161
  62. HIV-1 infection is restricted more efficiently by rhesus monkey TRIM5alpha than by human TRIM5alpha PMID: 14985764
  63. Data show that lentivirus susceptibility factor 1 and Ref1 are species-specific variants of tripartite interaction motif 5alpha (TRIM5alpha), a cytoplasmic body component that can block infection by widely divergent retroviruses. PMID: 15249685
  64. Data show that the human and African green monkey (AGM) TRIM5alpha genes encode Ref1 and Lv1 antiretroviral activities, respectively. PMID: 15249687
  65. human, rhesus, and African green monkey Trim5alpha can restrict N-tropic murine leukemia virus PMID: 15249690
  66. TRIM5alpha is both necessary and sufficient for the restriction of N-MLV in human cells; B-MLV was resistant to TRIM5alpha-mediated restriction; species-specific variation in TRIM5alpha governs its ability to block infection by diverse retroviruses PMID: 15280539
  67. The Trim5alpha SPRY domain was found to be responsible for targeting HIV-1 restriction. PMID: 15649369
  68. the major determinant of anti-HIV-1 potency is the B30.2(SPRY) domain PMID: 15709033
  69. TRIM5alpha, potently restricted infection by N-tropic murine leukemia virus (N-MLV) and moderately restricted human immunodeficiency virus type 1 (HIV-1) infection PMID: 15767395
  70. heterologous expression of TRIM5alpha(rh) or TRIMCyp interferes with the anti-N-MLV activity of endogenous TRIM5alpha; data show TRIM5 orthologues form heteromultimers & C-terminal extensions alter virus recognition by multimers of these proteins PMID: 15919943
  71. The SPRY domain of TRIM5alpha, showed higher nonsynonymous/synonymous substitution ratios than the non-SPRY domain, indicating that the adaptive evolution of TRIM5alpha in primates might be an innate strategy developed in defending retrovirus infection PMID: 16226405
  72. results indicate that the transcription and protein synthesis of TRIM5alpha could be modulated by IFN, suggesting that TRIM5alpha may play a role in an IFN-induced antiviral state against retrovirus infection PMID: 16289103
  73. An SNP at the human TRIM5 locus was found to have a functional consequence on in vitro retroviral infection of B cells. PMID: 16401428
  74. TRIM5alpha levels are maintained by continuous synthesis and rapid proteasome-mediated degradation, imbalances in which result in the formation of pre-aggresomal cytoplasmic bodies; turnover of TRIM5alpha is not required for its antiretroviral activity PMID: 16472833
  75. concluded that Fv1 and TRIM5 can both recognize retroviral particles early after cell entry and before reverse transcription PMID: 16474118
  76. observed no effect of individual TRIM5alpha nonsynonymous mutations on the in vitro HIV-1 susceptibility of CD4(+) T cells PMID: 16474153
  77. promotes HIV-1 infectivity completely independent of cyclophilin A PMID: 16501094
  78. Mechanism of HIV-1 restriction by TRIM5 alpha involves engagement of the viral capsid by the restriction factor prior to completion of uncoating. PMID: 16624363
  79. the positive effects of Cyp A-CA binding on HIV-1 infectivity do not depend on human TRIM5alpha PMID: 16643975
  80. polymorphism in human TRIM5 may influence susceptibility to HIV-1 infection PMID: 16887163
  81. This study revealed the importance of all three variable regions of TIRM5alpha B30.2 domain for determining retrovirus restriction specificity. PMID: 16912305
  82. diverse TRIM5 proteins inhibit retroviral infection in multiple ways PMID: 16973579
  83. TRIM5alpha-mediated HIV-1 restriction is related to selective degradation of cytosolic capsid normally associated with productive viral entry PMID: 17028189
  84. results show that even a moderate over expression of wild-type TRIM5alpha in human cells (2 fold as determined by quantitative RT-PCR) confers substantial restriction to infection for HIV-1 but only a weaker restriction to infection for HIV-2 PMID: 17087820
  85. The expression of TRIM5alpha, but not Fv-1, specifically promoted the premature conversion of particulate N-MLV capsids within infected cells to soluble capsid proteins. PMID: 17135314
  86. investigation of properties & antiviral activities of six TRIM family members, including those closely related to TRIM5alpha; only TRIM5alpha exhibited potent antiretroviral activity; also TRIM5alpha was unique in other biochemical & genetic properties PMID: 17156811
  87. Data suggest that TRIM5 alpha cytoplasmic bodies are dynamic structures more consistent with a role in function or regulation rather than protein aggregates or inclusion bodies that represent dead-end static structures. PMID: 17392513
  88. H43Y has a very minor effect on anti-HIV-1 activity of TRIM5alpha, suggesting that this allele is immaterial, at least in HIV-1-infected Europeans and Asians. PMID: 17406861
  89. Some alterations in the TRIM5alpha B-box 2 domain apparently affect the orientation or conformation of the B30.2(SPRY) domain, influencing capsid recognition. PMID: 17543365
  90. TRIM5 alpha may have protected early humans from invasion by Pan troglodytes endogenous retrovirus PMID: 17588933
  91. TRIM5alpha plays an essential role in controlling both the initial retroviral exposure and the subsequent viral dissemination in vivo. PMID: 17609277
  92. TRIM5alpha-mediated retroviral restriction results from the direct binding of the antiviral PRYSPRY domain to the viral capsid in murine leukemia virus infection PMID: 18166079
  93. Polymorphisms in the Trim5 gene may influence the clinical course of HIV-1 infection underscoring the antiviral effect of Trim5alpha on HIV-1 in vivo. PMID: 18248091
  94. TRIM5alpha functions as a RING-finger-type E3 ubiquitin ligase both in vitro and in vivo and ubiquitinates itself in cooperation with E2 ubiquitin-conjugating enzyme UbcH5B. PMID: 18312418
  95. Engagement of a restriction-sensitive retrovirus core results in TRIM5alpha degradation by a proteasome-dependent mechanism. PMID: 18497858
  96. TRIM5alpha variants lacking the SPRY domain are useful for silencing TRIM5alpha activity. PMID: 18524394
  97. Thus, the range of retroviruses restricted by human TRIM5alpha can be increased by changes in the B30.2/SPRY domain, which also result in the ability to cause premature uncoating of the restricted retroviral capsid. PMID: 18586294
  98. TRIM5 alpha is implicated in IFN-induced anti-retroviral response in primate cells. PMID: 18613956
  99. The TRIM5alpha B-box 2 domain promotes cooperative binding to the retroviral capsid by mediating higher-order self-association. PMID: 18799578
  100. These results indicate that, in addition to CA-specific binding, the PRYSPRY domain possesses a second function, possibly binding of a cofactor, that is essential for retroviral restriction activity by TRIM5alpha. PMID: 19153241

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Subcellular Location Cytoplasm, Nucleus
Protein Families TRIM/RBCC family
Database Links

HGNC: 16276

OMIM: 608487

KEGG: hsa:85363

STRING: 9606.ENSP00000369373

UniGene: Hs.125300

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