WIPI2 Antibody

Code CSB-PA896519ESR1HU
Size US$299 How to order?
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  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA896519ESR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA896519ESR1HU at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) WIPI2 Polyclonal antibody
Uniprot No. Q9Y4P8
Target Names WIPI2
Alternative Names ATG18B antibody; Atg21 antibody; CGI 50 antibody; DKFZp434J154 antibody; DKFZp686P02188 antibody; FLJ12979 antibody; FLJ14217 antibody; FLJ42984 antibody; WD repeat domain phosphoinositide-interacting protein 2 antibody; WD repeat domain; phosphoinositide interacting 2 antibody; WD40 repeat protein interacting with phosphoinositides 2 antibody; WIPI 2 antibody; WIPI-2 antibody; WIPI2 antibody; WIPI2_HUMAN antibody; WIPI49 like protein 2 antibody; WIPI49-like protein 2 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human WD repeat domain phosphoinositide-interacting protein 2 protein (205-454AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.; Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella.
Gene References into Functions
  1. Data suggest that, in patients with diabetic kidney disease, urinary excretion of mRNAs for MAP1LC3A, WIPI2, and RB1CC1 is down-regulated as compared to healthy control subjects; these transcripts may serve as urinary autophagy biomarkers. (MAP1LC3A = microtubule associated protein 1 light chain 3; WIPI2 = WD repeat domain phosphoinositide-interacting protein 2; RB1CC1 = RB1 inducible coiled-coil 1) PMID: 28760651
  2. Here the authors show that recruitment of WIPI2, itself essential for anti-bacterial autophagy, is dependent on the localization of catalytically active TBK1 to the vicinity of cytosolic bacteria. PMID: 27370208
  3. The specific autophagosomal localization of both WIPI1 and WIPI2 (refered to as WIPI puncta) has been employed to assess autophagy using fluorescence microscopy methods, such as confocal and live-cell video microscopy PMID: 25462558
  4. Data suggest WIPI1/WIPI2 co-localize with microtubule-associated light chain 3 and autophagy related proteins 2/14L, participate in biogenesis of phagosomes, autophagy, and mobilization of lipids to/from intracellular droplets. [review-like article] PMID: 25233424
  5. WIPI-1 and WIPI-2 are functionally required in mediating the PI3P signal at the onset of autophagy in NB4 cells. PMID: 24991767
  6. Data suggest that WIPI2b directly interacts with dimer of ATG16L1 (autophagy related 16-like 1) and this interaction is linked to production of phosphatidylinositol 3-phosphate in endoplasmic reticulum triggered by autophagosome formation. [REVIEW] PMID: 25233411
  7. WIPI2b binds the membrane surrounding Salmonella and recruits the Atg12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella. PMID: 24954904
  8. A role for WIPI2 in the progression of omegasomes into autophagosomes, is reported. PMID: 20505359
  9. Freeze-fracture replica immunolabelling reveals WD-repeat protein interacting with phosphoinositides 1 and 2 (WIPI-1 and WIPI-2) as membrane components of autophagosomes and the plasma membrane (PM). PMID: 21564513
  10. WIPI2 is a phosphatidylinsitol-3-phosphate binding protein required for starvation induced autophagy. PMID: 20505359

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Subcellular Location Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families WD repeat SVP1 family
Tissue Specificity Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.
Database Links

HGNC: 32225

OMIM: 609225

KEGG: hsa:26100

STRING: 9606.ENSP00000288828

UniGene: Hs.122363

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