WNK3 Antibody

Datasheet

Code	CSB-PA861176LA01HU
Size	US$166
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Image	Immunofluorescence staining of 293 cells with CSB-PA861176LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) WNK3 Polyclonal antibody

Uniprot No.

Q9BYP7

Target Names

WNK3

Alternative Names

KIAA1566 antibody; PRKWNK 3 antibody; PRKWNK3 antibody; Protein kinase lysine deficient 3 antibody; Protein kinase lysine-deficient 3 antibody; Protein kinase with no lysine 3 antibody; Serine/threonine protein kinase WNK3 antibody; Serine/threonine-protein kinase WNK3 antibody; WNK 3 antibody; WNK lysine deficient protein kinase 3 antibody; Wnk3 antibody; WNK3_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Serine/threonine-protein kinase WNK3 protein (1018-1181AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The WNK3 Antibody (Product code: CSB-PA861176LA01HU) is Non-conjugated. For WNK3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA861176LB01HU	WNK3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA861176LC01HU	WNK3 Antibody, FITC conjugated
Biotin	CSB-PA861176LD01HU	WNK3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.; Isoform 1, isoform 2, isoform 3 and isoform 4 stimulate the activity of SLC12A1, SLC12A2 and SLC12A3 and inhibit the activity of SLC12A4, SLC12A5, SLC12A6 and SLC12A7. According to PubMed:19470686, isoform 1 inhibits the activity of SLC12A3.

Gene References into Functions

Data show that WNK lysine deficient protein kinase 3 protein (WNK3) interacts with NCC and increases the Na-Cl cotransporter (NCC) expression on the cell membrane and in cytoplasm together. PMID: 27378340
WNK3 enhances NCC protein expression by increasing NCC synthesis via an ERK 1/2-dependent signaling pathway PMID: 27467688
WNK3 knockout mice exhibited significantly decreased infarct volume in a transgenic model of stroke and brain damage. PMID: 26069258
There may be a novel role for WNK3 on the renal Na+-Cl-cotransporter (NCC) expression at the plasma membrane, an effect apparently independent of the SPAK kinase and the aldosterone-SGK1 pathway. PMID: 24920754
LINGO-1 potentiates neuronal apoptosis, likely by inhibiting WNK3 kinase activity. PMID: 23482566
WNK3 modulates intracellular Cl(-) concentration and regulatory volume decrease in HEK293 cells. PMID: 22864523
Analysis of the data showed that WNK3 is an essential regulator of NKCC1 and that WNK3 activates NKCC1-mediated ion transport necessary for cell volume changes associated with cell invasion. PMID: 21813709
OXSR1 and WNK3 transcripts were substantially overexpressed in subjects with schizophrenia relative to comparison subjects. PMID: 20819979
WNK3 lies within the critical linkage interval for several human monogenic disorders, including X-linked mental retardation. The function of mammalian WNK3 kinase remains to be investigated. PMID: 15194194
WNK3 is involved in promoting cell survival by a mechanism at the level of procaspase-3 activation. PMID: 16501604
Results report that WNK3, another member of the WNK kinase family expressed by distal tubule cells, interacts with WNK4 and WNK1 to regulate NCC in both human kidney cells and Xenopus oocytes. PMID: 17975670
The positive effect of WNK3 on NCC also requires its catalytic activity PMID: 18701621
WNK3 is a positive regulator of the transcellular Ca2+ transport pathway (TRPV5 and TRPV6). PMID: 18768590
The C-terminal motifs contributed by exons 18 and 22 play an important role in the actions of WNK3 isoforms on NCCT. PMID: 19470686

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Subcellular Location

Cytoplasm.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, WNK subfamily

Tissue Specificity

Expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen. Isoform 1 is brain-specific. Isoform 3

Database Links

HGNC: 14543

OMIM: 300358

KEGG: hsa:65267

STRING: 9606.ENSP00000346667

UniGene: Hs.92423

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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