WSS1 Antibody

Code CSB-PA334485LA01SVG
Size US$166
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) WSS1 Polyclonal antibody
Uniprot No.
Target Names
WSS1
Alternative Names
WSS1 antibody; YHR134WDNA-dependent metalloprotease WSS1 antibody; EC 3.4.24.- antibody; DNA damage response protein WSS1 antibody; SUMO-dependent isopeptidase WSS1 antibody; Weak suppressor of SMT3 protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae
Immunogen
Recombinant Saccharomyces cerevisiae DNA-dependent metalloprotease WSS1 protein (1-269AA)
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated

The WSS1 Antibody (Product code: CSB-PA334485LA01SVG) is Non-conjugated. For WSS1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA334485LB01SVG WSS1 Antibody, HRP conjugated ELISA
FITC CSB-PA334485LC01SVG WSS1 Antibody, FITC conjugated
Biotin CSB-PA334485LD01SVG WSS1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Metalloendopeptidase that acts selectively on DNA-binding proteins. DNA is needed to bring the protease and substrates together to enable proteolysis. Involved in the repair of toxic DNA-protein cross-links (DPCs) such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesions or DPCs induced by reactive compounds such as formaldehyde. Involved in DNA damage response and processing of stalled or collapsed replication forks by removing the covalently trapped TOP1 from chromatin. DPC proteolysis enables the repair of the lesions via downstream DNA repair pathways. May be recruited to DPCs via the SUMOylation of substrate proteins at damaged DNA sites.
Gene References into Functions
  1. e show that SPRTN is a DNA-dependent mammalian protease required for resolving cytotoxic DNA-protein crosslinks (DPCs)- a function that had only been attributed to the metalloprotease Wss1 in budding yeast. We provide genetic evidence that SPRTN and Wss1 function distinctly in vivo to resolve DPCs PMID: 27852435
  2. Data suggest that Wss1 contributes to survival of DNA-protein crosslink (DPC)-harboring cells by acting on DPCs proteolytically. Wss1 enables replication of DPC-containing DNA and thus promotes genome integrity. PMID: 24998930
  3. Involement of WSS1 in the DNA damage response. PMID: 15598824
  4. In dividing cells, Wss1 is exclusively present in the mother cell. PMID: 18336552
Subcellular Location
Nucleus. Note=Present in a single sharp spot near the nuclear membrane, distinct from the spindle pole bodies and nucleolus. In dividing cells, the spot is exclusively present in the mother cell.
Protein Families
Peptidase M3 family, WSS1-like metalloprotease (WLM) subfamily
Database Links

KEGG: sce:YHR134W

STRING: 4932.YHR134W

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