ascl1a Antibody

Code CSB-PA852654LA01DIL
Size US$166
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) ascl1a Polyclonal antibody
Uniprot No.
Target Names
ascl1a
Alternative Names
ascl1a antibody; ash antibody; ash1a antibody; asha antibody; piaAchaete-scute homolog 1a antibody; Zash-1a antibody; Pituitary-absent protein antibody
Raised in
Rabbit
Species Reactivity
Zebrafish
Immunogen
Recombinant Zebrafish Achaete-scute homolog 1a protein (1-196AA)
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated

The ascl1a Antibody (Product code: CSB-PA852654LA01DIL) is Non-conjugated. For ascl1a Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA852654LB01DIL ascl1a Antibody, HRP conjugated ELISA
FITC CSB-PA852654LC01DIL ascl1a Antibody, FITC conjugated
Biotin CSB-PA852654LD01DIL ascl1a Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Transcriptional regulator. May mediate transcription activation by binding to the E box-containing promoter. Involved in neurogenesis. Required for the development of neurons in the epiphysis, acting partially redundantly with neurog1 and downstream of flh. Involved in maintaining rhombomere boundaries in the hindbrain, probably via up-regulation of delta expression. Also involved in pituitary development; required cell-autonomously in adenohypophyseal cells for endocrine differentiation and for survival of a subset of cells.
Gene References into Functions
  1. dlx1a/2a act downstream of ascl1a but upstream of dlx5a/dlx6a and gad1b to activate GABAergic specification PMID: 23747543
  2. bHLH transcription factor Ascl1a is essential for the specification of the intestinal secretory cells and mediates Notch signaling in the zebrafish intestine PMID: 23352790
  3. Loss of ascl1a prevents secretory cell differentiation within the zebrafish intestinal epithelium resulting in a loss of distal intestinal motility PMID: 23353550
  4. Data show that injury-dependent induction of Ascl1a suppressed expression of the Wnt signaling inhibitor, Dkk, and induced expression of the Wnt ligand, Wnt4a. PMID: 21911394
  5. The Ascl1a regulates Muller glia dedifferentiation and retinal regeneration through a Lin-28-dependent, let-7 microRNA signalling pathway. PMID: 20935637
  6. Expressed in the postsembryonic zebrafish brain PMID: 12638130
  7. ash1a and ngn1 function in parallel redundant pathways to regulate neurogenesis downstream of flh PMID: 12702659
  8. Ascl1a might act downstream of diencephalic fibroblast growth factor 3 (Fgf3) signaling to mediate some of the effects of Fgf3 on the developing adenohypophysis PMID: 16481349
  9. Cellular correlates of proneural gene(ash1a) expression in the regenerating zebrafish retina. PMID: 17822581
  10. ascl1a is essential for retina regeneration: within 4 h after retinal injury, ascl1a is induced in Muller glia. PMID: 18234889

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Subcellular Location
Nucleus.
Tissue Specificity
In the 24 hours embryo, expressed in the dorsal hindbrain in two bilaterally symmetrical lines of cells marking the boundary between the alar and basal plates, and ventrally in rhombomere 1 near the floor plate. Also expressed in embryonic adenohypophysis
Database Links

KEGG: dre:30466

STRING: 7955.ENSDARP00000056004

UniGene: Dr.314

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