Product Details

Full Product Name

Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) tal1 Polyclonal antibody

Uniprot No.

O93507

Target Names

tal1

Alternative Names

tal1 antibody; scl antibody; tal-1 antibody; si:ch211-135j1.3 antibody; T-cell acute lymphocytic leukemia protein 1 homolog antibody; TAL-1 antibody; Stem cell protein antibody; zSCL antibody

Raised in

Rabbit

Species Reactivity

Zebrafish

Immunogen

Recombinant Zebrafish T-cell acute lymphocytic leukemia protein 1 homolog protein (1-324AA)

Immunogen Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Conjugate

FITC

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Transcription factor that plays a pivotal role in hemopoietic and endothelial development, acting synergistically with lmo2 and downstream of clo. Specifies mesodermal precursors to a hemangioblast cell fate. Hemangioblasts are bipotential precursors of blood and endothelium, and in the absence of hemopoietic induction cues such as gata1, tal1/scl-lmo2-induced hemangioblasts differentiate into endothelial cells. Isoform alpha and isoform beta are redundant for the initiation of primitive hemopoiesis but have distinct roles in the regulation of primitive erythroid differentiation and definitive hemopoietic stem cell specification, most likely due to differences in expression levels. Specification of definitive hemopoietic stem cells requires isoform beta. DNA binding is required for erythroid maturation, but not for its other hemopoietic functions. Endothelial roles include development of the dorsal aorta, the site of definitive hemopoiesis in the embryo. Required for angiogenesis but not angioblast specification. Has an additional role in endocardium formation during heart development. May play a role in central nervous system development.

Gene References into Functions

The latter data suggest that Stil play a role in retina cell proliferation through the Shh signal transduction pathway. PMID: 24469449
Tal1 has distinct roles in regulating the formation of endocardial intercellular junctions. PMID: 24075907
Data suggest that a defined hemogenic endothelial population preset by scl-beta supports the deterministic emergence of HSCs, and unravel the cellular mechanisms by which scl isoforms regulate HSC development. PMID: 24046317
Our studies establish a new connection between RA and scl during development that may participate in stem cell self-renewal and hematopoietic differentiation PMID: 20410509
Ets1-related protein plays a critical role in the initiation of definitive hematopoiesis by controlling the expression of 2 stem cell leukemia (scl) isoforms (scl-alpha and scl-beta) in angioblasts. PMID: 20185582
in the absence of inducers of erythroid or myeloid haematopoiesis, Scl/Tal1-Lmo2-induced haemangioblasts differentiate into endothelial cells PMID: 14602685
Zebrafish scl has a role in hematopoietic and endothelial development, downstream of hemangioblast development. PMID: 15617691
scl is especially critical for the development of arteries where adult hematopoietic stem cells emerge, implicating scl in the formation of hemogenic endothelium PMID: 15644413
Scl participates in multiple processes requiring different levels and functions PMID: 16210319
While scl-alpha and -beta are redundant for the initiation of primitive hematopoiesis, these two isoforms exert distinct functions in the regulation of primitive erythroid differentiation and definitive hematopoietic stem cell specification. PMID: 17472439
In germline-transgenic zebrafish generated by inserting GFP into the first coding exon of scl, GFP expression completely recapitulates the endogenous expression of scl in blood, endothelium and CNS. PMID: 17559829
Scl/Tal1 is an indispensable component of the molecular hierarchy that controls endocardium morphogenesis. PMID: 17722983
These results demonstrate that scl is an important mediator of the ability of AML1-ETO to reprogram hematopoietic cell fate decisions, suggesting that scl may be an important contributor to AML1-ETO-associated leukemia. PMID: 18156164

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in hemopoietic and endothelial lineages. Isoform beta emerges first, expressing in the entire anterior and posterior lateral mesoderm (ALM and PLM respectively), and in the ventral wall of the dorsal aorta, where definitive hemopoiesis begins. I

Database Links

KEGG: dre:30766

STRING: 7955.ENSDARP00000077498

UniGene: Dr.75812