Code | CSB-BP358651MVH |
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Size | $528 |
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The gene responsible for the Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) hspX protein (2-144aa) is cloned into a plasmid vector to obtain recombinant plasmid, which is then transformed into baculovirus cells. baculovirus cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to endure a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. The protein carries a C-terminal 9xHis tag. Post-expression, affinity purification is employed to isolate and purify the recombinant Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) hspX protein from the cell lysate. The resulting recombinant protein is subjected to denaturing SDS-PAGE, allowing for an estimation of its purity, surpassing 85%.
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