Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

vvhA

Uniprot No.

P19247

Research Area

others

Alternative Names

vvhA; VV2_0404; Cytolysin

Species

Vibrio vulnificus (strain CMCP6)

Source

E.coli

Expression Region

21-471aa

Target Protein Sequence

QEYVPIVEKPIYITSSKIKCVLHTSGDFNATRDWCNAGASIDVRVNVAQMRSVQSATSDGFTPDAKIVRFTVDADKPGTGIHLVNELQQDHSWFQSWANRRTYIGPFASSYDLWVKPVSGYTPKKARDLPQNENKNYQHRDTYGYSIGINGKVGAEVNKDGPKVGGEVSGSFTYNYSKTLVFDTKDYRINNRSSLSDFDISFEREFGECDELRRQELGCYFTAAHWGSGWVFDKTKFNPISYSNFKPNYDVLYEAPVSETGVTDFEMGVKLNYRARFGTVLPSALFSVYGSAGSSTNSSTVKQRIRIDWNHPLFEAEAHVTLQSLSNNDLCLDVYGENGDKTVAGGSVNGWSCHGSWNQVWGLDKEERYRSRVASDRCLTVNADKTLTVEQCGANLAQKWYWEGDKLISRYVDGNNTRYLLNIVGGRNVQVTPENEANQARWKPTLQQVKL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

55.8 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The synthesis of Recombinant V. vulnificus vvhA protein began with the isolation of vvhA gene from the native genome. The next step was to amplify the vvhA gene by inserting the vvhA gene into an expression vector and then introducing the vector into the selected host cell, E.coli. Significant amounts of recombinant V. vulnificus vvhA protein were produced by the host only when expression genes were added. For the purpose of protein purification, the vector contains N-terminal 10xHis tag & C-terminal Myc tag to the vvhA DNA sequence. The purity of this protein is 85%+ determined by SDS-PAGE.

vvhA (also known as VV2_0404) is a gene encoding a protein named cytolysin in Vibrio vulnificus. The protein encoded by this gene belongs to HlyA hemolysin family. It has been proposed as a virulence factor with a molecular weight of 51kDa. This protein is specifically toxic to individual cells. It is involved in hemolysis in other organism, cytolysis and virulence.

Customer Reviews and Q&A

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■ Q&A

Firstly which vector system is used in this cytolysin protein recombinant production and which restriction sites have been used?
secondly if I order liquid form, would it be stable at -20 degree temperature for a longer time or should I order it in a lyophilised form for longer stability and activity?
thirdly the protein that will come to me will be myc tag free and histidine tag free or not?

The vector system used in this cytolysin protein recombinant production is pET21a and Thrombin site is in the N-terminal.
If the customer has demand for liquid form, we will add the corresponding 20-50% glycerol as a protective agent to enhance its stability at -20℃. The shelf life of liquid-form protein will be 6 months around at -20℃/-80℃. The shelf life of lyophilized form is 12 months around at -20℃/-80℃.
For the activity, we haven't expressed this protein/ have not tested its activity, so its activity is not 100% guaranteed.
If you are interested in other tag, please feel free to contact us again.

Target Background

Function

Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture by mechanisms not clearly defined.

Protein Families

HlyA hemolysin family

Database Links

KEGG: vvu:VV2_0404

Code	CSB-EP323085VFI
Abbreviation	Recombinant Vibrio vulnificus vvhA protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Vibrio vulnificus Cytolysin (vvhA)

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