Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
epiALantibiotic epidermin
Species
Staphylococcus epidermidis
Expression Region
31-52aa
Target Protein Sequence
IASKFICTPGCAKTGSFNSYCC
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-KSI-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
In the production of recombinant S. epidermidis epiA protein, the gene for epiA (E.coli) was cloned into a vector and expressed as epiA protein in E.coli. The plasmids with the copy of epiA, or the expression vector, were often used to enhance gene expression. Every step of production was undergone with a strict QC system. N-terminal 6xHis-KSI tag was used in the process. The purity is 85% determined by SDS-PAGE.
The Staphylococcus epidermidis genes encoding the His-tag-labelled epidermin precursor peptide EpiA. The 52-amino acid prepeptide EpiA consists of an N-terminal leader peptide with amino acids - 30 to - 1 and a C-terminal pro-epidermin with amino acids + 1 to + 22. EpiA is post-translationally converted to the mature antibiotic by dehydration of serine and threonine residues, sulfide bridge formation, oxidative decarboxylation at the C-terminus and removal of the leader peptide. enzymes involved in epidermin biosynthesis. Studies identified that EpiA was expressed in Escherichia coli using a malE-epiA fusion. The identity of purified EpiA was confirmed by ion spray mass spectrometry and amino acid sequencing. For EpiA detection, anti-EpiA antisera were raised. Upon prolonged incubation, factor Xa not only cleaved EpiA from the fusion protein, but also less efficiently cleaved EpiA internally between R-1 and I + 1.
