Recombinant AP-2 complex subunit mu (dpy-23)

Code CSB-YP001872CXY
MSDS
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Source Yeast
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Code CSB-EP001872CXY
MSDS
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Source E.coli
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Code CSB-EP001872CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001872CXY
MSDS
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Source Baculovirus
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Code CSB-MP001872CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dpy-23
Uniprot No.
Alternative Names
dpy-23; ap50; R160.1AP-2 complex subunit mu; Clathrin assembly protein complex 2 mu medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; Mu2-adaptin; Plasma membrane adaptor AP-2 50 kDa protein; Protein dumpy-23
Species
Caenorhabditis elegans
Expression Region
1-441
Target Protein Sequence
MIGGLFVYNH KGEVLISRIY RDDVTRNAVD AFRVNVIHAR QQVRSPVTNM ARTSFFHVKR GNVWICAVTR QNVNAAMVFE FLKRFADTMQ SYFGKLNEEN VKNNFVLIYE LLDEILDFGY PQNTDPGVLK TFITQQGVRT ADAPVPVTKE EQSQITSQVT GQIGWRREGI KYRRNELFLD VIEYVNLLMN QQGQVLSAHV AGKVAMKSYL SGMPECKFGI NDKITIEGKS KPGSDDPNKA SRAAVAIDDC QFHQCVKLTK FETEHAISFI PPDGEYELMR YRTTKDIQLP FRVIPLVREV SRNKMEVKVV VKSNFKPSLL AQKLEVRIPT PPNTSGVQLI CMKGKAKYKA GENAIVWKIK RMAGMKESQI SAEIDLLSTG NVEKKKWNRP PVSMNFEVPF APSGLKVRYL KVFEPKLNYS DHDVIKWVRY IGRSGLYETR C
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. AP50 is a subunit of the plasma membrane adaptor. Essential wnt/egl-20 signaling protein that functions in wnt/egl-20-producing cells. Required for the AP-2 complex-mediated endocytosis of membrane proteins including wntless homolog mig-14 in egl-20-producing cells. During development, regulates the migration of HSN neurons and the left and right Q neuroblasts (QL and QR, respectively) and their descendants, possibly through hox gene and wnt/egl-20 gene target mab-5, and plays a role in establishing ALM and PLM neuronal cell polarity. Required for the asymmetric divisions of V5 cells.
Gene References into Functions
  1. AP2 complex can function as two semi-independent hemicomplexes contributing to synaptic vesicle endocytosis. PMID: 23482940
  2. Levels of GluR GLR-1 decreased at synapses in ventral nerve cord (VNC) of Caenorhabditis elegans with mutations in AP2 subunits APM-2/mu2, APA-2/alpha or APS-2/sigma2. Genetic analyses suggest that APM-2/mu2 acts upstream of GLR-1 endocytosis in VNC. PMID: 25788288
  3. Mu2 adaptin/dpy-23 facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans. PMID: 19047463
Subcellular Location
Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.
Protein Families
Adaptor complexes medium subunit family
Tissue Specificity
Brain, heart, lung, liver, testis and spleen.
Database Links

KEGG: cel:CELE_R160.1

STRING: 6239.R160.1a

UniGene: Cel.6847

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