Recombinant Abnormal cell lineage protein 44 (lin-44)

Code CSB-YP634642CXY
MSDS
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Source Yeast
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Code CSB-EP634642CXY
MSDS
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Source E.coli
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Code CSB-EP634642CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP634642CXY
MSDS
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Source Baculovirus
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Code CSB-MP634642CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lin-44
Uniprot No.
Alternative Names
lin-44; E01A2.3Abnormal cell lineage protein 44; Wnt protein
Species
Caenorhabditis elegans
Expression Region
26-348
Target Protein Sequence
TNEIP TISGAPAGKI VQPPKPNILK QGCPSDLLHS RALRSIQLAC RTHPATVISA FEGVQEGLQN CANRLRFQQW DCSEAGNIMH DPPLLRQGFR ESSLIWALSS ASAAWGVATA CAQGWIDDCA CNNQMGQNEY EFGGCTHGVQ HGITASRKLL TKVGAVNTLL RKVEKHNLKA GRLAIKKTLI SSCKCHGVSG SCQQKTCWKR TATLEHITDY LVEKYARAKL YTDDSVVKTT DLIYLEASPD VCKAKSVAGR VCAWRNETHT QGDCDRLCCG NGFSIRHEVV RVKCDCEFVW CCNLVCKDCI QHRWISTCNG TPPKSLIF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Affects male tail development, vulval precursor cell specification and egg laying. Involved in morphogenesis by influencing polarity of asymmetric cell divisions of the B, U, and F cells in the male, and the T cell in males and hermaphrodites. Controls spindle orientation in B-gamma cell division during male copulatory spicule development. Involved in specification of the P7.p lineage during vulval development. Has a role in providing polarity and default lin-17 localization in axon development and positioning of neuromuscular synapses in DA9 regions by negatively regulating synaptogenesis. Plays a role in motorneuron development by promoting the extension of the anterior neurite of ventral D-type GABAergic motorneurons along the anterior-posterior axis of the ventral nerve cord.
Gene References into Functions
  1. The Wnt ligand lin-44 contributes to the early stages of gastrulation in parallel to sdn-1, but in a genetic pathway with ptp-3. PMID: 25938228
  2. In sensory neurons, the posteriorly enriched LIN-44/Wnt acts as a repellent to promote anteriorly directed neurite outgrowth through the LIN-17/Frizzled receptor, instead of controlling neuronal polarity as previously thought. PMID: 26460008
  3. our results strongly suggest that functions of at least four Wnts (lin-44, cwn-1, cwn-2, and egl-20) determine the polarity orientation of seam cells PMID: 22022276
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Wnt family
Tissue Specificity
Expressed in the tail hypodermis.
Database Links

KEGG: cel:CELE_E01A2.3

STRING: 6239.E01A2.3a

UniGene: Cel.19749

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