Recombinant African swine fever virus Virus attachment protein p12 (Pret-110), partial

Code CSB-YP315474AYG1
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP315474AYG1
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP315474AYG1-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP315474AYG1
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP315474AYG1
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pret-110
Uniprot No.
Alternative Names
Pret-110; Virus attachment protein p12; Protein p12
Species
African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

CSB-EP315474AYG Recombinant African swine fever Virus attachment protein p12 (Pret-110)
Could you please provide sequence, pricing, availability and tag information for all available sizes?
Have you received any customer feedback about the activity of this protein?
Thank you.

A:
Thanks for your inquiry.
This is a transmembrane protein, we can only provide the full length protein expressed by the cell free expression system and we can provide the partial protein expressed by the regular four expression systems.
Full length protein:
Recombinant African swine fever virus Virus attachment protein p12 (Pret-110)
CSB-CF315474AYG
Expression Region: 1-61aa; Full length.
Tag information:Tag type will be determined during the manufacturing process. (The expected tag is N-terminal 6xHis-SUMO-tagged)
Sequence:

MALDGSSGGGSNVETLLIVAIIVVIMAIMLYYFWWMPRQQKKCSKAEECTCNNGSCSLKTS


Partial protein:
Recombinant African swine fever virus Virus attachment protein p12 (Pret-110),partial
CSB-YP315474AYG1 >> Yeast
CSB-EP315474AYG1 >> E.coli
CSB-BP315474AYG1 >> Baculovirus
CSB-MP315474AYG1 >> Mammalian cell
Expression Region: 37-61aa; Fragment at the C-terminal.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows:
YP, BP, MP: N-terminal 6xHis-tagged; EP: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged.
Sequence:

PRQQKKCSKAEECTCNNGSCSLKTS



Regarding the activity. sorry, we haven't tested its activity and also haven't received the feedback about the activity from other customers.
Since we adpot affinity chromatography for purification, it's purified under very mild condition, in theory, it should be active, we recommend you to choose a small size to have a try.

Target Background

Function
Virus attachment protein.
Subcellular Location
Virion membrane; Single-pass membrane protein.
Protein Families
Asfivirus attachment protein p12 family
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