Recombinant Alcohol-sensitive RING finger protein 1

Code CSB-YP312397
MSDS
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Source Yeast
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Code CSB-EP312397
MSDS
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Source E.coli
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Code CSB-EP312397-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP312397
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP312397
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ASR1
Uniprot No.
Alternative Names
ASR1; YPR093C; Alcohol-sensitive RING finger protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-288
Target Protein Sequence
MEECPICLAD DQEGEQFGCL NVCGHKFHLN CIREWHKYSI NLKCPICRVE STHLEVGEGQ HALSINLKMG FMIKNAIDYV GAETTNERNE DDTGEQDQEI EFLSERLRGT LVMDTIKIIQ CSICGDTDVS RLSLYCQDCE AIYHETCLRG LACEVGDRNT WQECTDCRSN ALLELRMGAI SSQLASYDSR NSMIFAGELR DKHSVKTQQM YEQIRNAKHK IQMHVRRALD RYPLPLLRFK DAYKHVNKQV SRKLYRLSDN KYLPDQYDYD SLARTGVHTE LLIYCHDE
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for tolerance to alcohol.
Gene References into Functions
  1. A novel role for Asr1p in regulating cell cycle mediated by septin-dependent assembly in yeast.Asr1 involved in actin cytoskeleton influence the assembly of septin ring. PMID: 25646695
  2. a role of the Asr1p E3 ligase activity in coping with stress is proposed. PMID: 21726536
  3. Thus, when taken together, the present results suggest that Asr1 is involved in the response to ethanol stress in the yeast S. cerevisiae. PMID: 21193817
  4. it was concluded that Asr1 is not required and nor important for tolerance to alcohol stress PMID: 16391921
  5. Asr1 is a RING finger ubiquitin-ligase that binds directly to RNA polymerase II via the carboxyl-terminal domain of the largest subunit of the enzyme. PMID: 19064926
  6. Deletion of ASR1 leads to sensitivity in growth on medium containing alcohol or detergent, consistent with a function of Asr1p in alcohol-related signaling. PMID: 15117954

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Subcellular Location
Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Accumulates in the nucleus specifically upon alcohol stress. Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
Database Links

KEGG: sce:YPR093C

STRING: 4932.YPR093C

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