Recombinant Arabidopsis thaliana UDP-glycosyltransferase 89C1 (UGT89C1)

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Code CSB-YP873303DOA
Size $436
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP873303DOA could indicate that this peptide derived from Yeast-expressed Arabidopsis thaliana (Mouse-ear cress) UGT89C1.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP873303DOA could indicate that this peptide derived from Yeast-expressed Arabidopsis thaliana (Mouse-ear cress) UGT89C1.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
UGT89C1
Uniprot No.
Research Area
Others
Alternative Names
UGT89C1; At1g06000; T21E18.5; Flavonol 7-O-rhamnosyltransferase; EC 2.4.1.-; UDP-glycosyltransferase 89C1; UDP-rhamnose: flavonol 7-O-rhamnosyltransferase
Species
Arabidopsis thaliana (Mouse-ear cress)
Source
Yeast
Expression Region
1-435aa
Target Protein Sequence
MTTTTTKKPHVLVIPFPQSGHMVPHLDLTHQILLRGATVTVLVTPKNSSYLDALRSLHSPEHFKTLILPFPSHPCIPSGVESLQQLPLEAIVHMFDALSRLHDPLVDFLSRQPPSDLPDAILGSSFLSPWINKVADAFSIKSISFLPINAHSISVMWAQEDRSFFNDLETATTESYGLVINSFYDLEPEFVETVKTRFLNHHRIWTVGPLLPFKAGVDRGGQSSIPPAKVSAWLDSCPEDNSVVYVGFGSQIRLTAEQTAALAAALEKSSVRFIWAVRDAAKKVNSSDNSVEEDVIPAGFEERVKEKGLVIRGWAPQTMILEHRAVGSYLTHLGWGSVLEGMVGGVMLLAWPMQADHFFNTTLIVDKLRAAVRVGENRDSVPDSDKLARILAESAREDLPERVTLMKLREKAMEAIKEGGSSYKNLDELVAEMCL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
50.6 kDa
Protein Length
Full length
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Flavonol 7-O-rhamnosyltransferase that catalyzes the transfer of rhamnose from UDP-rhamnose to the 7-OH position of 3-O-glycosylated flavonols, such as kaempferol 3-O-rhamnoside, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, quercetin 3-O-galactoside, quercetin 3-O-rhamnoside and isorhamnetin 3-O-glucoside. Is able to glycosylate the flavonols quercetin and kaempferol to yield quercetin 7-O-rhamnoside and kaempferol 7-O-rhamnoside. Shows a strict specificity for UDP-rhamnose as sugar donor. Does not act on 3-O-glycosylated anthocyanins. The accumulation of kaempferol 3-O-rhamnoside-7-O-rhamnoside inhibits basipetal auxin transport, which influences auxin distribution and plant organ development.
Gene References into Functions
  1. Several rol1-2 suppressor mutants of the 7-O-rhamnosyltransferase locus UGT89C1 were identified. Levels of auxin conjugates and catabolites are strongly increased in the ugt89c1 mutant background. PMID: 26742840
Protein Families
UDP-glycosyltransferase family
Tissue Specificity
Highly expressed in floral buds. Expressed in stems, leaves and flowers. Expressed at low levels in roots and siliques. Expressed on the adaxial side of cotyledons and emerging leaves, in trichomes, root columella cells, and the late elongation/early diff
Database Links

KEGG: ath:AT1G06000

STRING: 3702.AT1G06000.1

UniGene: At.28159

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