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Recombinant Arabidopsis thaliana UDP-glycosyltransferase 89C1 (UGT89C1)

Recombinant Arabidopsis thaliana UDP-glycosyltransferase 89C1 (UGT89C1)

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Code	CSB-EP873303DOA
Abbreviation	Recombinant Mouse-ear cress UGT89C1 protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

UGT89C1

Uniprot No.

Q9LNE6

Research Area

Others

Alternative Names

UGT89C1; At1g06000; T21E18.5; Flavonol 7-O-rhamnosyltransferase; EC 2.4.1.-; UDP-glycosyltransferase 89C1; UDP-rhamnose: flavonol 7-O-rhamnosyltransferase

Species

Arabidopsis thaliana (Mouse-ear cress)

Source

E.coli

Expression Region

1-435aa

Target Protein Sequence

MTTTTTKKPHVLVIPFPQSGHMVPHLDLTHQILLRGATVTVLVTPKNSSYLDALRSLHSPEHFKTLILPFPSHPCIPSGVESLQQLPLEAIVHMFDALSRLHDPLVDFLSRQPPSDLPDAILGSSFLSPWINKVADAFSIKSISFLPINAHSISVMWAQEDRSFFNDLETATTESYGLVINSFYDLEPEFVETVKTRFLNHHRIWTVGPLLPFKAGVDRGGQSSIPPAKVSAWLDSCPEDNSVVYVGFGSQIRLTAEQTAALAAALEKSSVRFIWAVRDAAKKVNSSDNSVEEDVIPAGFEERVKEKGLVIRGWAPQTMILEHRAVGSYLTHLGWGSVLEGMVGGVMLLAWPMQADHFFNTTLIVDKLRAAVRVGENRDSVPDSDKLARILAESAREDLPERVTLMKLREKAMEAIKEGGSSYKNLDELVAEMCL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

51.6 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Arabidopsis thaliana (Mouse-ear cress) UGT89C1 protein expression in e.coli cells requires the insertion of a DNA fragment encoding the Arabidopsis thaliana (Mouse-ear cress) UGT89C1 protein (1-435aa) into a plasmid vector and the transferral of this vector into e.coli cells. The positive cells are screened, cultured, and then induced to express the UGT89C1 protein. The protein carries a N-terminal 10xHis tag. The cells are lysed to harvest the recombinant Arabidopsis thaliana (Mouse-ear cress) UGT89C1 protein, which is purified through affinity purification and then detected by SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of this recombinant Arabidopsis thaliana (Mouse-ear cress) UGT89C1 protein is over 85%.

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Target Background

Function

Flavonol 7-O-rhamnosyltransferase that catalyzes the transfer of rhamnose from UDP-rhamnose to the 7-OH position of 3-O-glycosylated flavonols, such as kaempferol 3-O-rhamnoside, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, quercetin 3-O-galactoside, quercetin 3-O-rhamnoside and isorhamnetin 3-O-glucoside. Is able to glycosylate the flavonols quercetin and kaempferol to yield quercetin 7-O-rhamnoside and kaempferol 7-O-rhamnoside. Shows a strict specificity for UDP-rhamnose as sugar donor. Does not act on 3-O-glycosylated anthocyanins. The accumulation of kaempferol 3-O-rhamnoside-7-O-rhamnoside inhibits basipetal auxin transport, which influences auxin distribution and plant organ development.

Gene References into Functions

Several rol1-2 suppressor mutants of the 7-O-rhamnosyltransferase locus UGT89C1 were identified. Levels of auxin conjugates and catabolites are strongly increased in the ugt89c1 mutant background. PMID: 26742840

Protein Families

UDP-glycosyltransferase family

Tissue Specificity

Highly expressed in floral buds. Expressed in stems, leaves and flowers. Expressed at low levels in roots and siliques. Expressed on the adaxial side of cotyledons and emerging leaves, in trichomes, root columella cells, and the late elongation/early diff

Database Links

KEGG: ath:AT1G06000

STRING: 3702.AT1G06000.1

UniGene: At.28159

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