Recombinant Arabidopsis thaliana 14-3-3-like protein GF14 psi (GRF3)

Code CSB-YP331592DOA
MSDS
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Source Yeast
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Code CSB-EP331592DOA
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Source E.coli
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Code CSB-EP331592DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP331592DOA
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Source Baculovirus
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Code CSB-MP331592DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GRF3
Uniprot No.
Alternative Names
GRF3; RCI1A; At5g38480; MXI10.2114-3-3-like protein GF14 psi; General regulatory factor 3; Protein RARE COLD INDUCIBLE 1A
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-255
Target Protein Sequence
MSTREENVYM AKLAEQAERY EEMVEFMEKV AKTVDVEELS VEERNLLSVA YKNVIGARRA SWRIISSIEQ KEESKGNEDH VAIIKDYRGK IESELSKICD GILNVLEAHL IPSASPAESK VFYLKMKGDY HRYLAEFKAG AERKEAAEST LVAYKSASDI ATAELAPTHP IRLGLALNFS VFYYEILNSP DRACSLAKQA FDDAIAELDT LGEESYKDST LIMQLLRDNL TLWTSDMTDE AGDEIKEASK PDGAE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes. Involved in the regulation of nutrient metabolism. Reciprocal negative transcription regulation of miR396. Negative regulator of constitutive freezing tolerance and cold acclimation by controlling cold-induced gene expression partially through an ethylene (ET)-dependent pathway; prevents ethylene (ET) biosynthesis, probably by binding 1-aminocyclopropane-1-carboxylate synthases (ACS) to reduce their stability, thus contributing to establish adequate ET levels under both standard and low-temperature conditions.
Gene References into Functions
  1. RCI1A connects the low-temperature response with ET biosynthesis to modulate constitutive freezing tolerance and cold acclimation in Arabidopsis. PMID: 25122152
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
14-3-3 family
Database Links

KEGG: ath:AT5G38480

STRING: 3702.AT5G38480.1

UniGene: At.22344

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